In vitro Investigation on the Biodegradability and Biocompatibility of Genipin Cross-linked Porcine Acellular Dermal Matrix with Intrinsic Fluorescence
In vitro Investigation on the Biodegradability and Biocompatibility of Genipin Cross-linked Porcine Acellular Dermal Matrix with Intrinsic Fluorescence
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京尼平交联猪脱细胞真皮基质固有荧光的体外生物降解性和生物相容性研究
DOI：
10.1021/am302272k
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发表时间：
2013-01-23
影响因子：
9.5
通讯作者：
Wang, Yingjun
中科院分区：
文献类型：
--
作者：
Qiu, Jichuan;Li, Jianhua;Wang, Yingjun
猪脱细胞真皮基质（PADM）作为一种生物相容性好、生物活性高的天然组织工程支架材料，由于其强度低、易降解等缺点，限制了其在组织再生中的应用。在这里，纯化的PADM改性的无毒交联剂（京尼平），以提高其机械性能和改善其耐酶降解。体外实验结果表明，京尼平交联的PADM的刚性得到提高，生物降解速率降低。细胞增殖实验结果表明京尼平交联反应不影响PADM的细胞相容性。此外，京尼平交联赋予可观察到的荧光，允许通过共聚焦激光扫描显微镜（CLSM）可视化支架的三维（3D）多孔结构和细胞分布。细胞核和细胞骨架的免疫组化染色表明MC 3 T3-E1前成骨细胞与交联PADM支架紧密粘附并均匀分布。本研究的结果表明，三维多孔京尼平交联PADM与固有荧光可能有更广泛的应用，组织工程支架，需要更高的机械刚度。
As a biocompatible and bioactive natural tissue engineering scaffold, porcine acellular dermal matrix (PADM) has limitations for the application in tissue regeneration due to its low strength and rapid biodegradation. Here, purified PADM was modified by a nontoxic cross-linker (genipin) to enhance its mechanical properties and improve its resistance to enzymatic degradation. In vitro testing results demonstrated that the stiffness of the genipin cross-linked PADM was improved and biodegradation rate was decreased. Results of cell proliferation assays showed that the cross-linking reaction by genipin did not undermine the cytocompatibility of PADM. Furthermore, genipin cross-linking imparted an observable fluorescence allowing visualization of the scaffold's three-dimensional (3D) porous structure and cell distribution by confocal laser scanning microscopy (CLSM). Immunostaining of the cell nuclei and cytoskeleton indicated that MC3T3-E1 preosteoblasts were tightly adhered to and uniformly distributed onto the crosslinked PADM scaffold. Results of this study suggest that the 3D porous genipin cross-linked PADM with intrinsic fluorescence may have broader applications for tissue engineering scaffolds where higher mechanical stiffness is needed.