Different Gene Expression Patterns in Human Papillary and Reticular Fibroblasts
Different Gene Expression Patterns in Human Papillary and Reticular Fibroblasts
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DOI：
10.1038/jid.2012.192
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发表时间：
2012-11-01
影响因子：
6.5
通讯作者：
El Ghalbzouri, Abdoelwaheb
中科院分区：
文献类型：
--
作者：
Janson, David G.;Saintigny, Gaelle;El Ghalbzouri, Abdoelwaheb
真皮包含两个不同的层次：乳头层和网状层。对来自这些层次的成纤维细胞进行的体外培养表明它们是不同的。然而，目前还没有已知的用于区分这两种成纤维细胞亚型的分子标志物。我们对从乳头层和网状层真皮中分离出来的培养成纤维细胞进行了基因表达分析。总共发现有116个基因存在差异表达。其中，13个基因通过定量逆转录酶 - 聚合酶链反应分析得到验证，并且在单层培养中两个标志物在蛋白质水平上得到验证。三个标志物在体内皮肤切片中显示出差异表达。所确定的这两种成纤维细胞亚群的特征性标志物为对网状层成纤维细胞和乳头层成纤维细胞进行功能研究提供了有用的工具。
The dermis contains two distinct layers: the papillary and the reticular layers. In vitro cultures of the fibroblasts from these layers show that they are different. However, no molecular markers to differentiate between the two subtypes of fibroblasts are known. We performed gene expression analysis on cultured fibroblasts isolated from the papillary and reticular dermis. In all, 116 genes were found to be expressed differentially. Of these, 13 were validated by quantitative reverse transcriptase-PCR analysis and two markers could be validated at the protein level in monolayer cultures. Three markers showed differential expression in in vivo skin sections. The identified, characteristic markers of the two fibroblast subpopulations provide useful tools to perform functional studies on reticular and papillary fibroblasts.