Altered circadian clock gene expression in patients with schizophrenia
Altered circadian clock gene expression in patients with schizophrenia
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DOI：
10.1016/j.schres.2016.04.029
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发表时间：
2016-07-01
影响因子：
4.5
通讯作者：
Lundkvist, Gabriella B.
中科院分区：
文献类型：
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作者：
Johansson, Anne-Sofie;Owe-Larsson, Bjorn;Lundkvist, Gabriella B.
在几种精神疾病中已经报道了昼夜节律性受损。精神分裂症通常与异常的睡眠-觉醒周期和失眠有关。尚不清楚精神分裂症是否与分子节律紊乱有关。我们分别培养了来自慢性精神分裂症患者和健康对照的皮肤样本的成纤维细胞，并分析了48小时内时钟基因CLOCK、BMAL 1、PER 1、PER 2、ERM 1、ERM 2、REV-ERB α和DBP的昼夜表达。在从慢性精神分裂症患者获得的成纤维细胞中，我们发现与来自健康对照的细胞相比，ER 1和PER 2的节律性表达丧失。我们还评估了这些患者的睡眠质量，发现与健康对照组相比，他们中的大多数人睡眠不好。在另一个患者样本中，我们分析了首次精神病发作的精神分裂症患者的单核血细胞，发现CLOCK的表达减少，这些新的发现显示了精神分裂症分子钟的紊乱，对我们理解精神分裂症的分子生物学机制具有重要意义。在这种疾病中报告的异常节律。(C)© 2016 Elsevier B. V.版权所有。
Impaired circadian rhythmicity has been reported in several psychiatric disorders. Schizophrenia is commonly associated with aberrant sleep-wake cycles and insomnia. It is not known if schizophrenia is associated with disturbances in molecular rhythmicity. We cultured fibroblasts from skin samples obtained from patients with chronic schizophrenia and from healthy controls, respectively, and analyzed the circadian expression during 48 h of the clock genes CLOCK, BMAL1, PER1, PER2, CRY1, CRY2, REV-ERB alpha and DBP. In fibroblasts obtained from patients with chronic schizophrenia, we found a loss of rhythmic expression of CRY1 and PER2 compared to cells from healthy controls. We also estimated the sleep quality in these patients and found that most of them suffered from poor sleep in comparison with the healthy controls.In another patient sample, we analyzed mononuclear blood cells from patients with schizophrenia experiencing their first episode of psychosis, and found decreased expression of CLOCK, PER2 and CRY1 compared to blood cells from healthy controls.These novel findings show disturbances in the molecular clock in schizophrenia and have important implications in our understanding of the aberrant rhythms reported in this disease. (C) 2016 Elsevier B.V. All rights reserved.