Genetic mapping and molecular marker development for the gene Pre2 controlling purple grains in barley
Genetic mapping and molecular marker development for the gene Pre2 controlling purple grains in barley
复制标题
控制大麦紫色籽粒的基因 Pre2 的遗传图谱和分子标记开发
DOI：
10.1007/s10681-015-1593-y
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发表时间：
2016-03-01
期刊：
影响因子：
1.9
通讯作者：
Zhang, Guoping
中科院分区：
文献类型：
--
作者：
Jia, Qiaojun;Zhu, Jinghuan;Zhang, Guoping
大麦的紫色籽粒是由糊粉和果皮中的花青素引起的，该性状由一个或两个显性基因控制。在本研究中，我们利用余姚红大麦与AC ParkHill或ACCA的正反交，证明紫色籽粒是由单个显性基因控制的。在余姚红大麦与ACCA杂交组合中，籽粒颜色与穗型紧密连锁(受Vrs1控制)。紫粒基因位于2HL染色体上，位于SSR标记Bmag0125(1.2 cM)和GBMS244(2.8 cM)之间，距Vrs1约5.1 cM。通过扩增基于大麦EST和水稻同源基因的Pre2附近的多态基因座，实现了目标区域的标记富集化。其中四个新开发的标记被定位在Pre2基因区域。紫色外膜和果皮基因(Pre2)被精确定位在Indel标记PQJ1056(来自EST BM096525)和HvOs04g47170之间，遗传距离分别为0.3和0.1 cM。
Purple grains in barley are caused by the presence of anthocyanin in both aleurone and pericarp, and the trait is controlled by one or two dominant genes. In this study, we demonstrated that purple grain was controlled by a single dominant gene using reciprocal crosses between Yuyaohongdamai and AC Parkhill or ACCA. The close linkage between grain color and spike type (controlled by Vrs1) was found in the crosses between Yuyaohongdamai and ACCA. The purple-grain gene was mapped on chromosome 2HL between the SSR markers Bmag0125 (1.2 cM) and GBMS244 (2.8 cM), and was about 5.1 cM distal from Vrs1. The marker enrichment in the target region was carried out by amplifying barley EST- and rice homologous gene-based searching for polymorphic loci adjacent to Pre2. Four of the newly developed markers were mapped in the Pre2 gene region. The purple lemma and pericarp gene (Pre2) was then finely mapped between InDel marker PQJ1056 (from EST BM096525) and HvOs04g47170 with genetic distance of 0.3 and 0.1 cM, respectively.