Variation in susceptibility of Helicoverpa armigera (Hubner) and Helicoverpa punctigera (Wallengren) (Lepidoptera: Noctuidae) in Australia to two Bacillus thuringiensis toxins
Variation in susceptibility of Helicoverpa armigera (Hubner) and Helicoverpa punctigera (Wallengren) (Lepidoptera: Noctuidae) in Australia to two Bacillus thuringiensis toxins
复制标题
DOI：
10.1016/j.jip.2006.08.005
复制
发表时间：
2007-02-01
影响因子：
3.4
通讯作者：
Akhurst, Raymond J.
中科院分区：
文献类型：
--
作者：
Bird, Lisa J.;Akhurst, Raymond J.
测定了澳大利亚棉铃虫Helicoverpa armigera（Hubner）和棉铃虫Helicoverpa punctigera（Wallengren）对苏云金芽孢杆菌（Bt）Cry 1Ac和Cry 2Ab δ-内毒素敏感性的种内变异，以建立监测Bt棉使用后可能发生的变化的基线。H. armigera和H. punctigera是从主要从整个澳大利亚棉花带的商业农场收集的种群建立的。使用两种生物测定方法（表面处理和饲料掺入），通过测量死亡率（LC 50）和生长抑制（IC 50）的剂量反应，评价菌株的敏感性。结果表明，不同浓度的H. armigera（n = 17株）和H. punctigera（n = 12株）对Cry 1Ac的反应分别为4.6倍和3.2倍。LC_（50）的变化，H. armigera（n = 19株）和H. punctigera（n = 12株）对Cry 2Ab的敏感性分别为6.6和3.5倍。Cry 1Ac对3 ~ 4龄棉铃虫的生长抑制范围为100%~ 200%。棉铃虫（n = 15株）为3.6倍; Cry 2Ab对棉铃虫幼虫的生长抑制范围为新生幼虫至3龄幼虫。棉铃虫（13株）为4.3倍; punstigera（n = 12株）为6.1倍。还评估了两个年龄组（新生儿和3龄）的H. armigera和H.点。新生儿H。3龄幼虫对Bt的敏感性与3龄幼虫相当或更高。新生儿H。棉铃虫对Cry 2Ab的敏感性高于3龄，对Cry 1Ac的敏感性低于3龄。两种生物测定方法的差异影响了物种对Bt毒素的相对敏感性，突出了生物测定方案标准化的必要性。(c)2006年爱思唯尔公司All rights reserved.
Intra-specific variation in susceptibility of Helicoverpa armigera (Hubner) and Helicoverpa punctigera (Wallengren) in Australia to the Cry1Ac and Cry2Ab delta-endotoxins from Bacillus thuringiensis (Berliner) (Bt) was determined to establish a baseline for monitoring changes that might occur with the use of Bt cotton. Strains of H. armigera and H. punctigera were established from populations collected primarily from commercial farms throughout the Australian cotton belts. Strains were evaluated for susceptibility using two bioassay methods (surface treatment and diet incorporation) by measuring the dose response for mortality (LC50) and growth inhibition (IC50). The variation in LC50 among H. armigera (n = 17 strains) and H. punctigera (n = 12 strains) in response to Cry1Ac was 4.6- and 3.2-fold, respectively. The variation in LC50, among H. armigera (n = 19 strains) and H. punctigera (n = 12 strains) to Cry2Ab was 6.6- and 3.5-fold, respectively. The range of Cry1Ac induced growth inhibition from the 3rd to 4th instar in H. armigera (n = 15 strains) was 3.6-fold and in H. punctigera (n = 13 strains) was 2.6-fold, while the range of Cry2Ab induced growth inhibition from neonate to 3rd instar in H. armigera (it = 13 strains) was 4.3-fold and in H. punctigera (n = 12 strains) was 6.1-fold. Variation in susceptibility was also evaluated for two age classes (neonates and 3rd instars) in laboratory strains of H. armigera and H. punctigera. Neonates of H. punctigera had the same or higher sensitivity to Bt than 3rd instars. Neonates of H. armigera were more sensitive to Cry2Ab than 3rd instars, while being less sensitive to Cry1Ac than 3rd instars. Differences in the two methods of bioassay used affected relative sensitivity of species to Bt toxins, highlighting the need to standardize bioassay protocols. (c) 2006 Elsevier Inc. All rights reserved.