Somatocrinin (growth hormone releasing factor) in vitro bioactivity; Ca++ involvement, cAMP mediated action and additivity of effect with PGE2.
Somatocrinin (growth hormone releasing factor) in vitro bioactivity; Ca++ involvement, cAMP mediated action and additivity of effect with PGE2.
复制标题
生长激素释放因子（生长激素释放因子）体外生物活性；
DOI：
10.1016/0006-291x(82)91762-4
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Brazeau,P;Ling,N;Esch,F;Böhlen,P;Mougin,C;Guillemin,R
由纯化的下丘脑GRF或天然或合成的肿瘤衍生的GRF诱导的GH的释放被CoCl 2的存在所拮抗;它被8Br所模拟。cAMP、IBMX、霍乱毒素、毛喉素，具有相同的最大作用（E max）。生长激素分泌素（GRF）刺激垂体细胞释放cAMP，同时释放GH。添加8Br。cAMP、IBMX、霍乱毒素或毛喉素对最大刺激剂量的GRF不增加反应，其保持GRF-E max。与这些结果相反，PGE 2释放GH的剂量-反应曲线与GRF不同，PGE 2+ GRF组合产生的Emax远大于单独使用任一种激动剂的Emax，显示出真正的加和性。建议使用生长激素配体（somatocrinin）这一名称来取代首字母缩略词GRF。
The release of GH induced by purified hypothalamic GRF or native or synthetic tumor-derived GRF is antagonized by the presence of CoCl 2; it is simulated by 8Br. cAMP, IBMX, cholera toxin, forskolin, with identical maximal effects (E max). Somatocrinin (GRF) stimulates the efflux of cAMP by the pituitary cells in parallel to the release of GH. Addition of either 8Br. cAMP, IBMX, cholera toxin or forskolin to a maximally stimulating dose of GRF does not increase the response which remains GRF-E max. In contradistinction with these results PGE 2 releases GH with a dose-response curve different from that of GRF, and the combination of PGE 2+ GRF produces an E max far greater than that due to either agonist alone; showing a true additivity. The name somatocrinin is proposed to replace the acronym GRF.