Transcriptome sequencing and development of an expression microarray platform for liver infection in adenovirus type 5-infected Syrian golden hamsters.
Transcriptome sequencing and development of an expression microarray platform for liver infection in adenovirus type 5-infected Syrian golden hamsters.
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DOI：
10.1016/j.virol.2015.07.024
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发表时间：
2015-11
期刊：
影响因子：
3.7
通讯作者：
Wold WS
中科院分区：
文献类型：
--
作者：
Ying B;Toth K;Spencer JF;Aurora R;Wold WS
叙利亚金黄仓鼠是一种有吸引力的动物，用于研究传染病和其他疾病。我们在这里报告的叙利亚仓鼠转录组的测序，组装和注释。我们包括从一个幼稚的仓鼠和脂多糖刺激的10个合并组织的转录本。我们的数据集确定了42，707个非冗余转录本，代表34，191个独特基因。基于转录组数据，我们生成了一个定制的微阵列，并使用这个新平台来研究静脉内腺病毒5型（Ad 5）感染后叙利亚仓鼠肝脏中的转录反应。我们发现，Ad 5感染引起肝脏转录调控的巨大变化，参与抗病毒反应的基因强烈上调，表明先天免疫反应在宿主防御Ad 5感染肝脏中发挥作用。本研究中开发的数据和新平台将促进这一重要动物模型的进一步发展。叙利亚仓鼠转录组; 42，707个转录本代表34，191个独特基因叙利亚仓鼠定制表达微阵列平台Ad 5静脉内感染叙利亚仓鼠肝脏Ad 5上调仓鼠肝脏基因参与先天性抗病毒反应.
The Syrian golden hamster is an attractive animal for research on infectious diseases and other diseases. We report here the sequencing, assembly, and annotation of the Syrian hamster transcriptome. We include transcripts from ten pooled tissues from a naïve hamster and one stimulated with lipopolysaccharide. Our data set identified 42,707 non-redundant transcripts, representing 34,191 unique genes. Based on the transcriptome data, we generated a custom microarray and used this new platform to investigate the transcriptional response in the Syrian hamster liver following intravenous adenovirus type 5 (Ad5) infection. We found that Ad5 infection caused a massive change in regulation of liver transcripts, with robust up-regulation of genes involved in the antiviral response, indicating that the innate immune response functions in the host defense against Ad5 infection of the liver. The data and novel platforms developed in this study will facilitate further development of this important animal model. Syrian hamster transcriptome; 42,707 transcripts representing 34,191 unique genes Syrian hamster custom expression microarray platform Ad5 intravenous infection of the Syrian hamster liver Ad5 upregulation of hamster liver genes involved in innate antiviral response.