Rice OsBT1 regulates seed dormancy through the glycometabolism pathway

Rice OsBT1 regulates seed dormancy through the glycometabolism pathway
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水稻 OsBT1 通过糖代谢途径调节种子休眠

DOI:
10.1016/j.plaphy.2020.03.055
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发表时间:
2020
影响因子:
6.5
通讯作者:
Wan Jianmin
Wan Jianmin
中科院分区:
生物学2区
文献类型:
--
作者:
Song Weihan;Hao Qixian;Cai Mengying;Wang Yihua;Zhu Xingjie;Liu Xi;Huang Yunshuai;Thanhliem Nguyen;Yang Chunyan;Yu Jiangfeng;Wu Hongming;Chen Liangming;Tian Yunlu;Jiang Ling;Wan Jianmin

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水稻(Oryza sativa L.)的种子休眠和发芽是复杂且重要的农艺性状,涉及许多生理过程和能量。从60Co辐射籼稻品种N22群体中筛选出的突变体h470,其休眠能力减弱,对赤霉素(GA)和脱落酸(ABA)不敏感。 h470 中 GA4 和 ABA 的水平高于野生型 (WT) 植物。通过mut-map和转基因克隆了h470中控制种子休眠的基因,并证实其编码ADP-葡萄糖转运蛋白。 Os02g0202400 (OsBT1) 中 1 bp 的缺失导致 h470 种子休眠能力较弱。代谢组学分析表明,h470 种子中的大多数糖成分均高于野生型。 .h470 中的突变影响糖代谢。
Seed dormancy and germination in rice (Oryza sativa L.) are complex and important agronomic traits that involve.a number of physiological processes and energy. A mutant named h470 selected from a60Co-radiated indica.cultivar N22 population had weakened dormancy that was insensitive to Gibberellin (GA) and Abscisic acid.(ABA). The levels of GA4 and ABA were higher in h470 than in wild-type (WT) plants. The gene controlling seed.dormancy in h470 was cloned by mut-map and transgenesis and confirmed to encode an ADP-glucose transporter.protein. A 1 bp deletion in Os02g0202400 (OsBT1) caused the weaker seed dormancy in h470. Metabolomics.analyses showed that most sugar components were higher in h470 seeds than the wild type. The mutation in.h470 affected glycometabolism.