Arabidopsis NRT1.2 interacts with the PHOSPHOLIPASE Dα1 (PLDα1) to positively regulate seed germination and seedling development in response to ABA treatment
Arabidopsis NRT1.2 interacts with the PHOSPHOLIPASE Dα1 (PLDα1) to positively regulate seed germination and seedling development in response to ABA treatment
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DOI：
10.1016/j.bbrc.2020.08.025
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发表时间：
2020-11-26
影响因子：
3.1
通讯作者：
Zuo, Kaijing
中科院分区：
文献类型：
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作者：
Li, Jianfu;Zhao, Chunyan;Zuo, Kaijing
NRT1.2在拟南芥中被鉴定为低亲和力的硝酸盐转运蛋白和脱落酸（阿坝）转运蛋白。在这项研究中，我们表明，NRT1.2正调控阿坝的反应在萌发和幼苗发育。在这些过程中，转基因拟南芥NRT1.2-过表达体表现出对阿坝的敏感性增加。qRT-PCR分析表明，在7天龄的幼苗中NRT 1.2的过量产生上调ABA响应基因的表达：ABI 1、ABI 2、ABI 3、ABI 4、ABI 5、RAB 18、RD 29 A和RD 29 B以及磷脂酶D α 1（PLD α 1）。与阿坝处理后的野生型相比，这些基因的表达在nrt 12突变体中被抑制。重要的是，双分子荧光互补分析表明，NRT 1.2与PLD α 1在质膜上相互作用。通过酵母双杂交（Y2 H）与基于交配的分裂泛素系统（MbSUS）的实验进一步证实了它们的相互作用。此外，遗传分析表明PLD α 1上位性地作用于NRT 1.2以影响阿坝信号传导。总之，我们的研究结果提供了详细的机制NRT1.2在ABA介导的种子萌发和幼苗发育。(C)2020爱思唯尔公司All rights reserved.
NRT1.2 has been characterized as a low-affinity nitrate transporter and an abscisic acid (ABA) transporter in Arabidopsis. In this study, we demonstrate that NRT1.2 positively regulated the ABA response during germination and seedling development. The transgenic Arabidopsis NRT1.2-over-expressionors showed increased sensitivity to ABA during these processes. qRT-PCR assays indicated that NRT1.2 overproduction in 7-days-old seedlings up-regulated the expression of ABA-responsive genes: ABI1, ABI2, ABI3, ABI4, ABI5, RAB18, RD29A, and RD29B and PHOSPHOLIPASE D alpha 1 (PLD alpha 1). The expression of these genes was suppressed in the nrt12 mutant in comparison with the wild type following ABA treatment. Importantly, bimolecular fluorescence complementation assays indicated that NRT1.2 interacts with PLD alpha 1 at the plasma membrane. Their interaction was further confirmed by using yeast two hybrid (Y2H) experiments with the mating-based split ubiquitin system (MbSUS). Moreover, genetic assays indicated that PLD alpha 1 acts epistatically on NRT1.2 to affect ABA signaling. Taken together, our results provide detailed mechanisms of NRT1.2 in ABA-mediated seed germination and seedling development. (C) 2020 Elsevier Inc. All rights reserved.