Evaluation of Early Osteochondral Defect Repair in a Rabbit Model Utilizing Fourier Transform-Infrared Imaging Spectroscopy, Magnetic Resonance Imaging, and Quantitative T2 Mapping
Evaluation of Early Osteochondral Defect Repair in a Rabbit Model Utilizing Fourier Transform-Infrared Imaging Spectroscopy, Magnetic Resonance Imaging, and Quantitative T2 Mapping
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DOI：
10.1089/ten.tec.2009.0020
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Pleshko, Nancy
中科院分区：
文献类型：
--
作者：
Kim, Minwook;Foo, Li F.;Pleshko, Nancy
背景：评估修复软骨的形态和基质成分是了解软骨修复的自然历史和潜在治疗方法有效性的关键一步。在本研究中，利用磁共振成像(MRI)、定量T2标测和傅立叶变换红外成像(FT-IRIS)对凝血酶多肽(TP-508)处理的兔骨软骨缺损模型进行了短期关节软骨修复(3周和6周)的评估。方法：在兔滑车沟内造成3 mm直径的骨软骨缺损，分别填充TP-508加聚乳酸微球和单纯聚乳酸微球(安慰剂)。分别于术后3周和6周对修复组织和邻近正常软骨进行评估。完整的膝关节通过磁共振成像评估修复形态，并通过定量T2图评估胶原的取向。结果与结论：修复组织的体积在两个时间点均无显著差异。在6周时，安慰剂修复组织显示出更长的T2值(p
Context: Evaluation of the morphology and matrix composition of repair cartilage is a critical step toward understanding the natural history of cartilage repair and efficacy of potential therapeutics. In the current study, short-term articular cartilage repair (3 and 6 weeks) was evaluated in a rabbit osteochondral defect model treated with thrombin peptide (TP-508) using magnetic resonance imaging (MRI), quantitative T2 mapping, and Fourier transform-infrared imaging spectroscopy (FT-IRIS).Methods: Three-mm-diameter osteochondral defects were made in the rabbit trochlear groove and filled with either TP-508 plus poly-lactoglycolidic acid microspheres or poly-lactoglycolidic acid microspheres alone (placebo). Repair tissue and adjacent normal cartilage were evaluated at 3 and 6 weeks postdefect creation. Intact knees were evaluated by magnetic resonance imaging for repair morphology, and with quantitative T2 mapping to assess collagen orientation. Histological sections were evaluated by FT-IRIS for parameters that reflect collagen quantity and quality, as well as proteoglycan (PG) content.Results and Conclusion: There was no significant difference in volume of repair tissue at either time point. At 6 weeks, placebo repair tissue demonstrated longer T2 values (p