Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation.
Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation.
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DOI：
10.2147/ijn.s136281
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发表时间：
2017
影响因子：
8
通讯作者：
Shao L
中科院分区：
文献类型：
--
作者：
Kang C;Wei L;Song B;Chen L;Liu J;Deng B;Pan X;Shao L
多孔钽（Ta）植入物具有高度的耐腐蚀性和生物相容性，并且它们具有比传统钛（Ti）植入物更好的初始稳定性。在负载磨损期间，沉积在多孔Ta植入物表面上的Ta纳米颗粒（Ta-NPs）不可避免地被释放并与植入物周围的成骨细胞直接接触。磨损碎屑可能影响细胞行为和植入物稳定性。然而，Ta-NPs与成骨细胞的相互作用尚未得到明确的研究。本研究旨在探讨Ta-NPs对细胞增殖的影响及其机制。细胞计数试剂盒-8（CCK-8）测定用于测量MC 3 T3-E1小鼠成骨细胞的细胞活力，并且显示Ta-NP处理可以增加细胞活力。共聚焦显微镜、Western blotting和透射电镜观察Ta-NPs诱导的细胞自噬，结果表明Ta-NPs诱导的细胞自噬表现为LC 3斑点阳性、LC 3-II高表达和自噬囊泡的超微结构。CCK-8测定显示，细胞活力分别通过应用自噬诱导剂和抑制剂而进一步增加和降低。此外，用自噬抑制剂3-甲基腺嘌呤（3-MA）预处理可抑制Ta-NP诱导的自噬。这些结果表明，Ta-NP可以促进细胞增殖，自噬诱导剂可以进一步加强这种作用，而自噬抑制剂可以减弱这种作用。总之，自噬参与了Ta-NP诱导的细胞增殖，并具有促进作用。
Porous tantalum (Ta) implants are highly corrosion resistant and biocompatible, and they possess significantly better initial stability than that of conventional titanium (Ti) implants. During loading wear, Ta nanoparticles (Ta-NPs) that were deposited on the surface of a porous Ta implant are inevitably released and come into direct contact with peri-implant osteoblasts. The wear debris may influence cell behavior and implant stabilization. However, the interaction of Ta-NPs with osteoblasts has not been clearly investigated. This study aimed to investigate the effect of Ta-NPs on cell proliferation and their underlying mechanism. The Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability of MC3T3-E1 mouse osteoblasts and showed that Ta-NP treatment could increase cell viability. Then, confocal microscopy, Western blotting, and transmission electron microscopy were used to confirm the autophagy induced by Ta-NPs, and evidence of autophagy induction was observed as positive LC3 puncta, high-LC3-II expression, and autophagic vesicle ultrastructures. The CCK-8 assay revealed that the cell viability was further increased and decreased by the application of an autophagy inducer and inhibitor, respectively. In addition, pre-treatment with autophagy inhibitor 3-methyladenine (3-MA) inhibited the Ta-NP-induced autophagy. These results indicate that the Ta-NPs can promote cell proliferation, that an autophagy inducer can further strengthen this effect and that an autophagy inhibitor can weaken this effect. In conclusion, autophagy was involved in Ta-NP-induced cell proliferation and had a promoting effect.