RXR agonist enhances the differentiation of cardiomyocytes derived from embryonic stem cells in serum-free conditions
RXR agonist enhances the differentiation of cardiomyocytes derived from embryonic stem cells in serum-free conditions
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DOI：
10.1016/j.bbrc.2005.05.202
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发表时间：
2005-08-12
影响因子：
3.1
通讯作者：
Asashima, M
中科院分区：
文献类型：
--
作者：
Honda, M;Hamazaki, TS;Asashima, M
来自视黄酸受体（RAR）和类维生素A X受体（RXR）的信号传导对于体内心血管形态发生是必需的。RAR和/或RXR信号传导还可以增强在血清存在下从鼠胚胎干（ES）细胞体外诱导心肌细胞。本研究在无血清的情况下，检测了与RXR特异性结合的RXR激动剂对小鼠ES细胞体外分化为心肌细胞的影响。在用RXR激动剂PA 024处理后，来自ES细胞的搏动胚状体样球（EBSs）的数量显著增加。相反，当用与RXR特异性结合并作为拮抗剂的PA 452处理EBSs时，搏动EBSs的数量以剂量依赖性方式减少。这些结果表明，RXR信号调节心肌细胞的数量在分化的ES细胞在体外，并可能在正常的发展。(c)2005年爱思唯尔公司All rights reserved.
Signaling from the retinoic acid receptors (RARs) and retinoid X receptors (RXRs) is essential for cardiovascular morphogenesis in vivo. RAR and/or RXR signaling can also enhance the in vitro induction of cardiomyocytes from murine embryonic stem (ES) cells in the presence of serum. The present study examined the effect of RXR agonist that was specifically bound to RXRs on the differentiation of mouse ES cells into cardiomyocytes in vitro in the absence of serum. The number of beating embryoid body-like spheres (EBSs) derived from the ES cells increased significantly following treatment with PA024, an RXR agonist. In contrast, when EBSs were treated with PA452, which was specifically bound to RXR and worked as an antagonist, the number of beating EBSs was decreased in a dose-dependent manner. These results suggest that RXR signaling regulates cardiomyocyte numbers during the differentiation of ES cells in vitro and probably in normal development. (c) 2005 Elsevier Inc. All rights reserved.