EXTENSIBLE AND LESS-EXTENSIBLE DOMAINS OF CONNECTIN FILAMENTS IN STRETCHED VERTEBRATE SKELETAL-MUSCLE SARCOMERES AS DETECTED BY IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY USING MONOCLONAL-ANTIBODIES
EXTENSIBLE AND LESS-EXTENSIBLE DOMAINS OF CONNECTIN FILAMENTS IN STRETCHED VERTEBRATE SKELETAL-MUSCLE SARCOMERES AS DETECTED BY IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY USING MONOCLONAL-ANTIBODIES
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DOI：
10.1093/oxfordjournals.jbchem.a122499
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发表时间：
1988-10-01
影响因子：
2.7
通讯作者：
MARUYAMA, K
中科院分区：
文献类型：
--
作者：
ITOH, Y;SUZUKI, T;MARUYAMA, K
针对连接蛋白（肌肉弹性蛋白）的两种单克隆抗体（3B 9和SM 1）与α-SMA和α-SMA均反应。和β-连接.免疫荧光研究显示，3B 9染色鸡胸肌肌原纤维的A带的两个边缘，并且在拉伸至3.5 μ m的肌节长度时保持原样。另一方面，SM 1染色的I带非常靠近A带的边缘，并且SM 1染色的条纹在伸展至3.5 μ m的肌节长度时显著移动。对青蛙半腱肌的免疫电子显微镜观察显示，在A带的边缘与3B 9结合的三个不同的条纹在拉伸至3.5 μ m时不移动。另一方面，I带中的两个SM 1染色的条带明显移动到与拉伸相同的程度。然而，当肌节被拉伸至4.0 μ m时，所有具有3B 9或SM 1的条纹消失，并且观察到抗体的扩散沉积。因此，它的结论是，连接蛋白丝在I带区比那些在A带的两个边缘更可伸展。
Two kinds of monoclonal antibodies (3B9 and SM1) against connectin, muscle elastic protein, reacted with both .alpha.- and .beta.-connections. Immunofluorescence studies revealed that 3B9 stained both edges of the A band of chicken breast muscle myofibrils and remained as such upon stretching to a sarcomere length of 3.5 .mu.m. On the other hand, SM1 stained the I band very close to the edges of the A band and the SM1-stained stripes moved considerably upon stretching to a sarcomere length of 3.5 .mu.m. Immunoelectron microscopic observations with frog semitendinous muscle revealed that three distinct stripes bound with 3B9 in the edges of the A band did not move on stretching up to 3.5 .mu.m. On the other hand, the two stripes stained with SM1 in the I band clearly moved to the same extent as the stretching. However, when a sarcomere was stretched to 4.0 .mu.m, all the stripes with 3B9 or SM1 disappeared and diffused deposits of the antibodies were observed. Thus it is concluded that connectin filaments in the I band region are more extensible than those at both edges of the A band.