Activated Pancreatic Enzyme and Pancreatic Stone Protein (PSP/reg) in Bile of Patients with Pancreaticobiliary Maljunction/Choledochal Cysts
Activated Pancreatic Enzyme and Pancreatic Stone Protein (PSP/reg) in Bile of Patients with Pancreaticobiliary Maljunction/Choledochal Cysts
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DOI：
10.1007/s10620-004-9599-7
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发表时间：
2004-11
影响因子：
3.1
通讯作者：
K. Ochiai;K. Kaneko;M. Kitagawa;H. Ando;T. Hayakawa
中科院分区：
文献类型：
--
作者：
K. Ochiai;K. Kaneko;M. Kitagawa;H. Ando;T. Hayakawa
胰胆交接不良/胆总管囊肿的症状是由共同通道中的蛋白质塞压缩导致的胆管和胰管阻塞引起的。然而，蛋白质栓形成的机制仍然未知。胰石蛋白（PSP）是慢性胰腺炎形成蛋白栓的关键蛋白。本文分析了13例胰胆管合流异常患者的胆汁和2例正常人的胆汁。检测各组大鼠胰腺酶活性和PSP浓度。胆管胆汁中PSP的平均浓度为76.9 ± 30.9 ng/mL，胆囊胆汁中PSP的平均浓度为76.9 ± 29.8 ng/mL。对照组未检测到PSP（P&<0.05）。在患者的胆管胆汁中，胰蛋白酶（原）在9例患者中可检测到，其中7例患者有激活的胰蛋白酶。在12名患者的胆囊胆汁中检测到胰蛋白酶（原），其中9名患者的胰蛋白酶已活化。在对照组中既未检测到活化的胰蛋白酶也未检测到胰蛋白酶原。胰胆管合流异常患者胆汁中同时含有活化胰蛋白酶和PSP。活化的胰蛋白酶切割可溶性PSP并产生不溶性PSP。胰胆管合流异常中的蛋白栓可能是由组装的不溶性PSP形成的。
Symptoms of pancreaticobiliary maljunction/choledochal cysts are caused by the obstruction of bile and pancreatic ducts due to protein plugs compacted in the common channel. However, the mechanism of protein plug formation remains unknown. Pancreatic stone protein (PSP) is reported to be a key protein to form protein plugs in chronic pancreatitis. Bile from 13 patients with pancreaticobiliary maljunction and bile from two normal controls were analyzed. Activity of pancreatic enzymes and the concentration of PSP were measured. The mean concentrations of PSP were 76.9 ± 30.9 ng/mL in the bile-duct bile, and 76.9 ± 29.8 ng/mL in the gallbladder bile. PSP was not detected in the controls (P&<0.05). In the bile-duct bile of the patients, trypsin(ogen) was detectable in nine patients, of which seven patients had activated trypsin. In the gallbladder bile, trypsin(ogen) was detectable in 12 patients, of which 9 patients had activated trypsin. Neither activated trypsin nor trypsinogen was detected in the controls. Bile in pancreaticobiliary maljunction patients contained both activated trypsin and PSP. Activated trypsin cleaves soluble PSP and creates insoluble PSP. Protein plugs in pancreaticobiliary maljunction may be formed by assembled insoluble PSP.