Differential expression of protein kinase C betaI (PKCbetaI) but not PKCalpha and PKCbetaII in the suprachiasmatic nucleus of selected house mouse lines, and the relationship to arginine-vasopressin.
Differential expression of protein kinase C betaI (PKCbetaI) but not PKCalpha and PKCbetaII in the suprachiasmatic nucleus of selected house mouse lines, and the relationship to arginine-vasopressin.
复制标题
选定家鼠品系的视交叉上核中蛋白激酶 C betaI (PKCbetaI) 的差异表达，但 PKCα 和 PKCbetaII 没有差异表达，以及与精氨酸加压素的关系。
DOI：
10.1016/s0006-8993(01)02821-9
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发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
VanderZee,EA
中科院分区：
文献类型：
--
作者：
Bult,A;Kobylk,ME;VanderZee,EA
视交叉上核（SCN）在哺乳动物昼夜节律控制中的功能意义已得到充分证明。然而，蛋白激酶 C (PKC) 介导的蛋白磷酸化的作用尚不清楚。我们报告了三种 Ca2+ 依赖性 PKC 亚型（α、βI、βII）在行为昼夜节律参数不同的选定家鼠品系的 SCN 内的免疫细胞化学定位。光密度测量显示，选择进行低水平筑巢行为的成年小鼠（小筑巢者）的 SCN 中 PKCβI 免疫染色比选择进行高水平筑巢行为的小鼠（大筑巢者）高出三倍多。在成年小筑巢者和大筑巢者之间，观察到 SCN 中含有 PKCβI 的细胞数量存在类似的两倍差异。未选择的对照线是中间的。十天大的幼崽在 SCN 中的 PKCβI 免疫染色显示小型和大型筑巢者之间存在相似的差异。 SCN 中的 PKCα 和 PKCβII 免疫染色在各系之间没有差异。下丘脑后前核 (LA) 和背侧海马角氨区 1 (CA1) 的所选细胞系中 PKCβI 免疫染色没有差异，并证实了所选细胞系中 SCN 中 PKCβI 免疫染色差异的特异性。这些发现的意义是在SCN中精氨酸-加压素（AVP）和光诱导的Fos表达谱系之间的差异、持续黑暗中对15分钟光脉冲的行为相延迟反应以及所表达的昼夜节律活动强度的测量的背景下讨论的。
The functional significance of the suprachiasmatic nucleus (SCN) in circadian rhythm control of mammals has been well documented. The role of protein phosphorylation mediated by protein kinase C (PKC), however, is not well known. We report the immunocytochemical localization of three Ca2+-dependent PKC isoforms (α, βI, βII) within the SCN of selected house mouse lines that differ in behavioral circadian rhythm parameters. Optical density measurements revealed that the adult mice selected for low levels of nest-building behavior (small nest-builders) had more than threefold higher PKCβI immunostaining in the SCN than the mice selected for high levels of nest-building behavior (big nest-builders). A similar twofold difference between the adult small and big nest-builders was observed for the number of PKCβI-containing cells in the SCN. The non-selected control lines were intermediate. Ten-day-old pups revealed similar differences in PKCβI immunostaining in the SCN between the small and big nest-builders. PKCα and PKCβII immunostaining in the SCN was not different among the lines. PKCβI immunostaining was not different among the selected lines in the lateroanterior hypothalamic nucleus (LA) and the cornu ammonis field 1 (CA1) of the dorsal hippocampus and confirms the specificity of the difference in PKCβI immunostaining in the SCN among the selected lines. The significance of these findings is discussed in the context of differences among the lines in arginine-vasopressin (AVP) and light-induced Fos expression in the SCN, behavioral phase-delay responses to 15-min light pulses in constant darkness, and measures of the strength of the circadian activity rhythm expressed.