SUGGESTIVE EVIDENCE THAT BLOCKING-ANTIBODIES OF TUMOR-BEARING INDIVIDUALS MAY BE ANTIGEN-ANTIBODY COMPLEXES (MOLONEY SARCOMA/METHYLCHOLANTHRENE-INDUCED/ADSORPTION/ELUTION)
SUGGESTIVE EVIDENCE THAT BLOCKING-ANTIBODIES OF TUMOR-BEARING INDIVIDUALS MAY BE ANTIGEN-ANTIBODY COMPLEXES (MOLONEY SARCOMA/METHYLCHOLANTHRENE-INDUCED/ADSORPTION/ELUTION)
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DOI：
10.1073/pnas.68.6.1372
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
HELLSTROM, KE
中科院分区：
文献类型：
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作者：
SJOGREN, HO;HELLSTROM, I;HELLSTROM, KE
携带Moloney病毒或甲基胆蒽诱导的进行性肉瘤的小鼠血清可以阻断对肿瘤特异性抗原免疫的淋巴细胞的细胞毒性作用。通过吸收含有不同类型肿瘤细胞的血清，可以特异性地去除阻断作用，并可以在低pH下从这些细胞中洗脱。如果保持低pH，则可以从洗脱液中分离出低分子量和高分子量的部分。如果将这些组分加入到靶细胞中45分钟，然后取出，这两种组分都不能阻断淋巴细胞介导的细胞毒性，而它们1:1的混合物具有特定的阻断作用。如果它们与淋巴细胞混合，孵育1小时，然后在整个2天的测试中与靶细胞和淋巴细胞孵育，低分子量部分以及混合物，而不是高分子量部分，具有阻断活性。这表明，定期测试的荷瘤动物血清中的阻断因子是一种抗原-抗体复合物，能够与靶细胞结合和/或与对其抗原免疫的淋巴细胞反应，从而阻断淋巴细胞的反应性；后一种反应被认为是暂时的。
Sera from mice carrying progressively growing sarcomas induced by Moloney virus or methylcholanthrene can block the cytotoxic effect of lymphocytes immune to the tumor-specific antigens of the respective neoplasms. The blocking effect can be specifically removed by absorbing sera with the respective types of tumor cells, and it can be recovered from these cells by elution at low pH. If the low pH is maintained, it is possible to separate out a low and a high molecular weight fraction from the eluates. If the fractions are added to the target cells for 45 minutes and then removed, neither of these fractions can block lymphocyte-mediated cytotoxicity, while a 1:1 mixture of them has a specific blocking effect. If they are admixed with the lymphocytes, incubated for 1 hr, and then allowed to incubate with the target cells and lymphocytes during the entire 2 days of the test, the low molecular weight fraction, as well as the mixture, but not the high molecular weight fraction, has a blocking activity.It is suggested that the blocking factor in sera from tumor-bearing animals, as regularly tested, is an antigen-antibody complex, capable of binding to the target cells and/or reacting with lymphocytes immune to their antigens, thus blocking the lymphocytes' reactivity; the latter reaction is postulated to be of a temporary nature.