Basement membrane-like matrix of teratocarcinoma-derived endodermal cells: presence of laminin and heparan sulfate in the matrix at points of attachment to cells.
Basement membrane-like matrix of teratocarcinoma-derived endodermal cells: presence of laminin and heparan sulfate in the matrix at points of attachment to cells.
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畸胎癌衍生的内胚层细胞的基底膜样基质：在细胞附着点的基质中存在层粘连蛋白和硫酸乙酰肝素。
DOI：
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发表时间：
1983
影响因子：
3.2
通讯作者：
I. Leivo
中科院分区：
文献类型：
--
作者：
I. Leivo
畸胎癌来源的内皮Pys-2细胞合成一种细胞外基质，其主要成分为基底膜分子层粘连蛋白、IV型胶原和硫酸乙酰肝素蛋白多糖(I.Leivo，K.Alitalo，L.Risteli，A.Vaheri，R.Timpl，J.Wartiovaara，Exp Cell res 137：15-23,1982)。本研究采用免疫铁蛋白技术，结合特异性抗体，对上述成分在纤维网络中的超微结构定位进行了研究。层粘连蛋白存在于邻近基底膜的基质网络和连接基质与细胞膜的突起基质纤维中。Ru红染色的对肝素酶敏感的10-20 nm颗粒通常出现在附着纤维和基质网络的交界处，或沿着附着纤维。基底膜硫酸乙酰肝素蛋白多糖的铁蛋白标记也有相应的分布。IV型胶原存在于基质网络中，但不存在于附着纤维中。结果表明，Pys-2细胞通过含有含有硫酸乙酰肝素颗粒的层粘连蛋白的纤维与其细胞周围基质相连。这些结果也可能与上皮细胞与基底膜的附着有关。
Teratocarcinoma-derived endodermal PYS-2 cells are known to synthesize an extracellular matrix containing the basement membrane molecules laminin, type IV collagen, and heparan sulfate proteoglycan as major constituents (I. Leivo, K. Alitalo, L. Risteli, A. Vaheri, R. Timpl, J. Wartiovaara, Exp Cell Res 137:15-23, 1982). Immunoferritin techniques with specific antibodies were used in the present study to define the ultrastructural localization of the above constituents in the fibrillar network. Laminin was detected in matrix network adjacent to the basal cell membrane and in protruding matrix fibrils that connect the matrix to the cell membrane. Ruthenium red-stainable heparinase-sensitive 10- to 20-nm particles were often present at the junction of the attachment fibrils and the matrix network, or along the attachment fibrils. A corresponding distribution of ferritin label was observed for basement membrane heparan sulfate proteoglycan. Type IV collagen was found in the matrix network but not in the attachment fibrils. The results suggest that the PYS-2 cells are connected to their pericellular matrix by fibrils containing laminin associated with heparan sulfate-containing particles. These results may also have relevance for the attachment of epithelial cells to basement membranes.
研究糖蛋白 GP-2 在体内肾小球内和体外培养的肾细胞株中的定位。
DOI：
10.1177/29.11.7033360
发表时间：
1981
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Oberley,TD;Chung,AE;Murphy-Ullrich,JE;Mosher,DF
通讯作者：
Mosher,DF