A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals.
A sensitive viral capture assay for detection of plasma viremia in HIV-infected individuals.
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一种灵敏的病毒捕获测定，用于检测 HIV 感染者的血浆病毒血症。
DOI：
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发表时间：
1992
影响因子：
1.5
通讯作者：
J. Allain
中科院分区：
文献类型：
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作者：
D. Henrard;W. F. Mehaffey;J. Allain
人类免疫缺陷病毒（HIV）在包膜HIV-1外膜蛋白和跨膜蛋白单克隆抗体的微颗粒上捕获病毒粒子后，在人免疫缺陷病毒（HIV）感染者的血清/血浆中检测到病毒。我们分析了从6名血清转化者、18名无症状者和12名艾滋病患者中获得的一系列样本。在所有（29/29）血清阳性样本中检测到HIV-1 RNA，在血清转化前立即检测到6个血清阴性样本。相比之下，在13/29（45%）的血清阳性样本中检测到HIV抗原。在血清转化前8-5个月的3份抗原阴性样本中检测到HIV-1 RNA，在抗原阳性前2天的另一份样本中检测到HIV-1 RNA。聚合酶链反应（PCR）信号强度与HIV抗原浓度呈平行关系。相反，血清阳性HIV抗原阴性样本的PCR信号较弱。在无症状、HIV抗原阴性个体的10/18（60%）样本和艾滋病患者的11/12（92%）样本中检测到HIV-1 RNA。病毒捕获方法可以提供一种敏感、特异和半定量的方法来检测感染所有阶段的循环HIV。
Human immunodeficiency virus was detected in the serum/plasma of individuals infected with human immunodeficiency virus (HIV) after capture of virions on microparticles coated with monoclonal antibodies to external and transmembrane proteins of HIV-1. We analyzed serial samples obtained from 6 individuals who seroconverted, 18 asymptomatic, and 12 AIDS patients. HIV-1 RNA was detected in all (29/29) seropositive samples and in 6 seronegative samples immediately preceding seroconversion. In contrast, HIV antigen was detected in 13/29 (45%) of seropositive samples. HIV-1 RNA was also detected in 3 antigen-negative samples from one individual 8-5 months prior to seroconversion and in one sample from another person 2 days before antigen positivity. The intensity of the polymerase chain reaction (PCR) signal paralleled the concentration of HIV antigen. Conversely, seropositive HIV antigen-negative samples gave a weaker PCR signal. HIV-1 RNA was detected in 10/18 (60%) samples from asymptomatic, HIV antigen-negative, individuals and in 11/12 (92%) specimens obtained from AIDS patients. The viral capture method may provide a sensitive, specific, and semiquantitative means of detecting circulating HIV at all stages of infection.
影响因子：
39.2
作者：
SCHNITTMAN, SM;GREENHOUSE, JJ;LANE, HC
通讯作者：
LANE, HC
影响因子：
158.5
作者：
HO, DD;MOUDGIL, T;ALAM, M
通讯作者：
ALAM, M
影响因子：
158.5
作者：
DAAR, ES;MOUDGIL, T;HO, DD
通讯作者：
HO, DD