Simultaneous Determination of Ascorbic Acid and Dehydroascorbic Acid by High Performance Liquid Chromatography
Simultaneous Determination of Ascorbic Acid and Dehydroascorbic Acid by High Performance Liquid Chromatography
复制标题
高效液相色谱法同时测定抗坏血酸和脱氢抗坏血酸
DOI：
10.2116/analsci.7.supple_125
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发表时间：
1991
影响因子：
1.6
通讯作者：
M. Hayashi
中科院分区：
文献类型：
--
作者：
Y. Yasui;M. Hayashi
It has been found that ascorbic acid (AA) and dehydro-ascorbic acid (DHAA) can be derivatized to the compounds having a maximum absorption wavelength of 3OOnm under alkaline conditions, and that der¡vatizati on of DHAA is accelerated in the presence of a reducing agent such as sodium borohydride. This reaction has been applied to post-column derivatization in high performance liquid chromatography (HPLC) for the simultaneous determination of AA and DHAA, In this system, AA in the range of 1ng 2 µ g and DHAA in the range of 2ng 2 µ g have been determined simultaneously with good reproducibility, C. V 1.5% (n = 10).
