Cloning and expression of the EP2 subtype of human receptors for prostaglandin E2.
Cloning and expression of the EP2 subtype of human receptors for prostaglandin E2.
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DOI：
10.1006/bbrc.1994.1142
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发表时间：
1994-02
影响因子：
3.1
通讯作者：
S. An;J. Yang;M. Xia;E. Goetzl
中科院分区：
文献类型：
--
作者：
S. An;J. Yang;M. Xia;E. Goetzl
前列腺素 E2 (PGE2) 是许多人体组织中的有效介质，可被三种不同的受体亚型（称为 EP1、EP2 和 EP3）识别。来自人肺文库的 cDNA 编码与小鼠 EP2 受体有 88% 同源性的 53 kDa 蛋白质。 COS-7细胞转染子中的人EP2受体以2.2nM的平均Kd和天然特异性结合[3H]-PGE2，并且转导细胞内环AMP浓度增加，但Ca++浓度不增加。大多数 EP2 受体 mRNA 存在于肺、肾、肠、腺体和免疫组织中，这与功能反应一致。
Prostaglandin E2 (PGE2) is a potent mediator in many human tissues, that is recognized by three distinct subtypes of receptors, designated EP1, EP2 and EP3. A cDNA from a human lung library encodes a 53 kDa protein of 88% homology with the mouse EP2 receptor. Human EP2 receptors in COS-7 cell transfectants bound [3H]-PGE2 with a mean Kd of 2.2 nM and native specificity, and transduced increases in the intra-cellular concentration of cyclic AMP, but not of Ca++. That most EP2 receptor mRNA is in lung, kidney, intestinal, glandular and immune tissues, is consistent with functional responses.