LabKey Server NAb: a tool for analyzing, visualizing and sharing results from neutralizing antibody assays.
LabKey Server NAb: a tool for analyzing, visualizing and sharing results from neutralizing antibody assays.
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DOI：
10.1186/1471-2172-12-33
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发表时间：
2011-05-27
期刊：
影响因子：
3
通讯作者：
Igra M
中科院分区：
文献类型：
--
作者：
Piehler B;Nelson EK;Eckels J;Ramsay S;Lum K;Wood B;Greene KM;Gao H;Seaman MS;Montefiori DC;Igra M
多种类型的检测试剂可灵敏地检测病毒特异性中和抗体。例如，HIV-1、SIV和SHIV的抗体中和程度可以在TZM-bl细胞系中通过感染后荧光素酶报告基因表达的程度来测量。过去，该标准品测定的中和曲线和滴度是使用Excel宏计算的。用新技术更新这样一个宏的所有实例可能会很麻烦，并且会在多个实验室团队之间引入不一致性。使用Excel还带来了集中存储、共享和关联原始数据文件和结果的挑战。我们介绍LabKey Server的NAb工具，用于组织、分析和安全共享中和抗体（NAb）检测的数据、文件和结果，包括基于酶的TZM-bl NAb检测。可定制的工具支持高通量实验，并包括一个图形化的板模板设计器，使研究人员能够快速适应新的板布局的计算。该工具基于发光测量值计算各血清稀释液的中和百分比，将一系列中和曲线拟合至滴定结果，并使用这些曲线估计基准稀释液的中和抗体滴度。结果、曲线可视化和原始数据文件存储在数据库中，并通过基于Web的安全界面共享。NAb结果可以基于样本标识符与其他数据源集成。通过更新文件夹安全设置，可以在发布后将结果公开。用于分析、存档和共享检测结果的标准化工具可以提高许多实验室获得的结果的重现性、可比性和可靠性。LabKey服务器及其NAb工具可在Apache 2.0许可证下在http://www.labkey.com上作为开源软件免费获得。HIV研究界的许多成员也可以使用Atlas Science Portal（https：//www.example.com）访问LabKey Server NAb工具，而无需安装软件。atlas.scharp.org Atlas是LabKey Server的安装。
Multiple types of assays allow sensitive detection of virus-specific neutralizing antibodies. For example, the extent of antibody neutralization of HIV-1, SIV and SHIV can be measured in the TZM-bl cell line through the degree of luciferase reporter gene expression after infection. In the past, neutralization curves and titers for this standard assay have been calculated using an Excel macro. Updating all instances of such a macro with new techniques can be unwieldy and introduce non-uniformity across multi-lab teams. Using Excel also poses challenges in centrally storing, sharing and associating raw data files and results. We present LabKey Server's NAb tool for organizing, analyzing and securely sharing data, files and results for neutralizing antibody (NAb) assays, including the luciferase-based TZM-bl NAb assay. The customizable tool supports high-throughput experiments and includes a graphical plate template designer, allowing researchers to quickly adapt calculations to new plate layouts. The tool calculates the percent neutralization for each serum dilution based on luminescence measurements, fits a range of neutralization curves to titration results and uses these curves to estimate the neutralizing antibody titers for benchmark dilutions. Results, curve visualizations and raw data files are stored in a database and shared through a secure, web-based interface. NAb results can be integrated with other data sources based on sample identifiers. It is simple to make results public after publication by updating folder security settings. Standardized tools for analyzing, archiving and sharing assay results can improve the reproducibility, comparability and reliability of results obtained across many labs. LabKey Server and its NAb tool are freely available as open source software at http://www.labkey.com under the Apache 2.0 license. Many members of the HIV research community can also access the LabKey Server NAb tool without installing the software by using the Atlas Science Portal (https://atlas.scharp.org). Atlas is an installation of LabKey Server.
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影响因子：
3
作者：
Nelson EK;Piehler B;Eckels J;Rauch A;Bellew M;Hussey P;Ramsay S;Nathe C;Lum K;Krouse K;Stearns D;Connolly B;Skillman T;Igra M
通讯作者：
Igra M
影响因子：
2.9
作者：
Gottschalk, PG;Dunn, JR
通讯作者：
Dunn, JR
影响因子：
3.7
作者：
Okuma, K;Nakamura, M;Matsuura, Y
通讯作者：
Matsuura, Y
影响因子：
2.2
作者：
Lam, Regina;Farrell, Rachel;Oger, Joel
通讯作者：
Oger, Joel
影响因子：
4.4
作者：
Rauch, A;Bellew, M;McIntosh, MW
通讯作者：
McIntosh, MW