Skeletal muscle transcriptomics identifies common pathways in nerve crush injury and ageing.
Skeletal muscle transcriptomics identifies common pathways in nerve crush injury and ageing.
复制标题
骨骼肌转录组学识别神经挤压损伤和衰老的共同途径。
DOI：
10.1186/s13395-021-00283-4
复制
发表时间：
2022-01-29
期刊：
影响因子：
4.9
通讯作者：
Jackson MJ
中科院分区：
文献类型：
--
作者：
Staunton CA;Owen ED;Hemmings K;Vasilaki A;McArdle A;Barrett-Jolley R;Jackson MJ
运动单位的重建包括反复去神经和重新神经支配，在一生中发生。这个过程的效率随着年龄的增长而下降，这是导致神经肌肉缺陷的原因之一。本研究通过建立C57BL/6J小鼠运动单位重塑模型，研究了腓神经损伤后肌肉中差异表达基因(DEG)的表达。在挤压后3 天分离肌肉总RNA，用Poly-A选择法构建文库，测序，并利用基因本体论和路径分析工具进行分析。与4~6月龄成年小鼠相比，26月龄小鼠的静止肌中有334个DEG，而成年小鼠的肌肉中也有这些DEG。腓骨挤压在成年小鼠肌肉中诱导出7133℃，在老年小鼠肌肉中诱导出699℃，但在直接比较老年和成年小鼠神经挤压肌肉时，只发现一种DEG(ZCCHC17)。这项分析揭示了肌肉反应的关键差异，这可能是老年小鼠神经损伤后修复能力减弱的基础。网上版载有补充材料，可在10.1186/s13395-021-00283-4查阅。
Motor unit remodelling involving repeated denervation and re-innervation occurs throughout life. The efficiency of this process declines with age contributing to neuromuscular deficits. This study investigated differentially expressed genes (DEG) in muscle following peroneal nerve crush to model motor unit remodelling in C57BL/6 J mice. Muscle RNA was isolated at 3 days post-crush, RNA libraries were generated using poly-A selection, sequenced and analysed using gene ontology and pathway tools. Three hundred thirty-four DEG were found in quiescent muscle from (26mnth) old compared with (4-6mnth) adult mice and these same DEG were present in muscle from adult mice following nerve crush. Peroneal crush induced 7133 DEG in muscles of adult and 699 DEG in muscles from old mice, although only one DEG (ZCCHC17) was found when directly comparing nerve-crushed muscles from old and adult mice. This analysis revealed key differences in muscle responses which may underlie the diminished ability of old mice to repair following nerve injury. The online version contains supplementary material available at 10.1186/s13395-021-00283-4.
登录
查看更多内容
影响因子：
7.8
作者：
Bhaskaran S;Pollock N;C Macpherson P;Ahn B;Piekarz KM;Staunton CA;Brown JL;Qaisar R;Vasilaki A;Richardson A;McArdle A;Jackson MJ;Brooks SV;Van Remmen H
通讯作者：
Van Remmen H
影响因子：
3.7
作者：
Chai RJ;Vukovic J;Dunlop S;Grounds MD;Shavlakadze T
通讯作者：
Shavlakadze T
影响因子：
5.5
作者：
Cheng, Ming;Nguyen, Mai-Huong;Koh, Timothy J.
通讯作者：
Koh, Timothy J.
影响因子：
2.7
作者：
Day, Kenneth;Shefer, Gabi;Yablonka-Reuveni, Zipora
通讯作者：
Yablonka-Reuveni, Zipora
影响因子：
9.2
作者：
Charles, P;Tait, S;Lubetzki, C
通讯作者：
Lubetzki, C