A Universal Paper-Based Electrochemical Sensor for Zero-Background Assay of Diverse Biomarkers
A Universal Paper-Based Electrochemical Sensor for Zero-Background Assay of Diverse Biomarkers
复制标题
用于多种生物标记物零背景测定的通用纸基电化学传感器
DOI：
10.1021/acsami.9b03860
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发表时间：
2019-05-01
影响因子：
9.5
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Liu, Xiaojuan;Li, Xiuyuan;Li, Feng
本文描述了一种通用的基于纸的电化学传感平台，该平台使用修饰有信号分子标记的DNA的纸和丝网印刷电极沿着目标识别溶液来实现对多种类型的生物标志物的检测。这些测定依赖于靶诱导的Mg 2+依赖性DNAzyme的合成，用于催化底物DNA从纸上切割，这已经通过分别使用miR-21的microRNA识别探针、碱性磷酸酶的磷酸化发夹探针和癌胚抗原测定的DNA适体得到证实。利用高特异性靶触发聚合/切口和DNA酶催化的信号放大的优点，本发明的测定能够在零背景下高度灵敏和选择性地检测这些靶。这些检测试剂盒还可用于检测加标血清样本中的靶标，证明了临床样本即时检测的潜力。
This paper describes a universal paper-based electrochemical sensing platform that uses a paper modified with signal molecule-labeled DNA and a screen-printed electrode along with target recognition solutions to achieve the detection of multiple types of biomarkers. These assays rely on the target-induced synthesis of Mg2+-dependent DNAzyme for catalyzing the cleavage of substrate DNA from paper, which have been demonstrated by using microRNA recognition probe for miR-21, a phosphorylated hairpin probe for alkaline phosphatase, and a DNA aptamer for carcinoembryonic antigen assays, respectively. Taking advantages of the high specific target-triggered polymerization/nicking and DNAzyme-catalyzed signal amplification, the present assays enable highly sensitive and selective detection of these targets with zero-background. These assays can also be applied to detect target in spiked serum samples, demonstrating the potential for point-of-care detection of clinical samples.