Abundance and diversity based on amoA genes of ammonia-oxidizing archaea and bacteria in ten wastewater treatment systems
Abundance and diversity based on amoA genes of ammonia-oxidizing archaea and bacteria in ten wastewater treatment systems
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基于 10 个废水处理系统中氨氧化古菌和细菌 amoA 基因的丰度和多样性
DOI：
10.1007/s00253-013-5428-2
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发表时间：
2014-04-01
影响因子：
5
通讯作者：
Peng, Yongzhen
中科院分区：
文献类型：
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作者：
Gao, Jingfeng;Luo, Xin;Peng, Yongzhen
采用聚合酶链反应（PCR）、克隆、测序和实时荧光定量PCR（qPCR）技术，对10个污水处理系统中氨氧化古菌（AOA）和细菌（AOB）的amoA基因丰度和多样性进行了研究。这十个WTS包括四个全尺寸的市政WTS，三个全尺寸的工业WTS和三个实验室规模的WTS。AOB在所有WTS中存在，而AOA在9个WTS中检测到。QPCR数据显示，AOBamoAbees（4.625 × 104-9.99 × 109 copies g− 1 sludge）在每个WTS中的数量超过AOAamoAbees（<检测限-1.90 × 107 copies g− 1 sludge），表明AOB可能比AOA在WTS中的氨氧化中发挥重要作用。有趣的是，发现AOA和AOB与厌氧氨氧化（anammox）细菌共存，在三个厌氧氨氧化WTS相对较高的丰度。在一个全面的工业废水处理系统中，出水氨氮高于进水氨氮，AOA和AOB都表现出较高的丰度。系统发育分析表明，亚硝化单胞菌属（Nitrosomonas）是10个WTS中最具优势的类群;欧洲亚硝化单胞菌群（Nitrosomonaseuropaeacluster）是主要的优势类群，其次是类亚硝化单胞菌群（Nitrosomonas-like clusters）和寡养亚硝化单胞菌群（Nitrosomonasoligotrophacluster）; AOB类群的多样性高于AOA类群。AOA分属于两个大类群：亚硝化球蛋白类群（Nitrososphaeracluster）和亚硝化球蛋白类群（Nitrosopumiluscluster），其中亚硝化球蛋白类群是各样品中的优势种，分布广泛。
The abundance and diversity ofamoAgenes of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were investigated in ten wastewater treatment systems (WTSs) by polymerase chain reaction (PCR), cloning, sequencing, and quantitative real-time PCR (qPCR). The ten WTSs included four full-scale municipal WTSs, three full-scale industrial WTSs, and three lab-scale WTSs. AOB were present in all the WTSs, whereas AOA were detected in nine WTSs. QPCR data showed that AOBamoAgenes (4.625 × 104–9.99 × 109copies g−1sludge) outnumbered AOAamoAgenes (<limit of detection–1.90 × 107copies g−1sludge) in each WTS, indicating that AOB may play an important role than AOA in ammonia oxidization in WTSs. Interestingly, it was found that AOA and AOB coexisted with anaerobic ammonia oxidation (anammox) bacteria in three anammox WTSs with relatively higher abundance. In a full-scale industrial WTS where effluent ammonia was higher than influent ammonia, both AOA and AOB showed higher abundance. The phylogenetic analysis of AOBamoAgenes showed that generaNitrosomonaswas the most dominant species in the ten WTSs;Nitrosomonas europaeacluster was the dominant major cluster, followed byNitrosomonas-like cluster andNitrosomonas oligotrophacluster; and AOB species showed higher diversity than AOA species. AOA were found to be affiliated with two major clusters:Nitrososphaeracluster andNitrosopumiluscluster.Nitrososphaeracluster was the most dominant species in different samples and distributed worldwide.