Increase in ovarian leukotrienes during hormonally induced ovulation in the rat.
Increase in ovarian leukotrienes during hormonally induced ovulation in the rat.
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大鼠激素诱导排卵期间卵巢白三烯增加。
DOI：
10.1152/ajpendo.1989.256.6.e753
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Okamura,H
中科院分区：
文献类型：
--
作者：
Espey,LL;Tanaka,N;Okamura,H
25 天大的 Wistar 大鼠在注射怀孕马血清促性腺激素（PMSG；皮下注射 10 IU）后 2 天，通过注射人绒毛膜促性腺激素（hCG；皮下注射 10 IU）来启动排卵过程。进入排卵过程 4 小时，白三烯 (LT) B4 增加 2 倍（P 小于 0.001），LT C4/D4/E4 增加 1.3 倍（P 小于 0.002）。到排卵时（施用 hCG 后 10-12 小时），两种类二十烷酸均降至 hCG 前水平。当动物在hCG前1小时用特定剂量的环加氧酶抑制剂吲哚美辛0.316mg/大鼠皮下治疗时，在hCG后4小时时，卵巢中LT B4和LTs C4/D4/E4的水平分别增加至210%（P小于0.01）和113%（P小于0.05），高于对照水平。与此同时，该剂量的吲哚美辛使卵巢前列腺素 (PG) E 和 F 分别降低 99%（P 小于 0.001）和 98%（P 小于 0.001），并使排卵率降低 76%（P 小于 0.001）。因此，该剂量的吲哚美辛似乎阻断了卵巢花生四烯酸向 PG 的转化，并将该底物分流至导致 LT 形成的脂氧合酶途径。总之，卵巢 LT 的适度增加是炎症反应的特征，因此，这些数据支持排卵生化事件类似于炎症过程的假设。
The ovulatory process was initiated in 25-day-old Wistar rats by injecting human chorionic gonadotropin (hCG; 10 IU sc) 2 days after the animals had been primed with pregnant mares serum gonadotropin (PMSG; 10 IU sc). By 4 h into the ovulatory process, leukotriene (LT) B4 increased 2-fold (P less than 0.001) and LTs C4/D4/E4 increased 1.3-fold (P less than 0.002). By the time of ovulation (10-12 h after the administration of hCG) both eicosanoids declined to their pre-hCG levels. When animals were treated with the cyclooxygenase inhibitor indomethacin at the specific dose of 0.316 mg/rat sc at 1 h before hCG, the ovarian levels of LT B4 and LTs C4/D4/E4 increased to 210% (P less than 0.01) and 113% (P less than 0.05), respectively, above the control levels at 4 h after hCG. Concomitantly, this dosage of indomethacin reduced ovarian prostaglandins (PGs) E and F by 99% (P less than 0.001) and 98% (P less than 0.001), respectively, and it reduced the ovulation rate by 76% (P less than 0.001). Thus it appears this dose of indomethacin blocked the conversion of ovarian arachidonic acid into PGs and shunted this substrate into the lipoxygenase pathways that lead to LT formation. In conclusion, the moderate increase in ovarian LTs is characteristic of inflammatory reactions, and, therefore, these data support the hypothesis that the biochemical events of ovulation resemble an inflammatory process.