Promotion of the self-renewal capacity of human acute leukemia cells by Wnt3A.
Promotion of the self-renewal capacity of human acute leukemia cells by Wnt3A.
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发表时间：
2008-09
影响因子：
2
通讯作者：
N. Kawaguchi-Ihara;I. Murohashi;N. Nara;S. Tohda
中科院分区：
文献类型：
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作者：
N. Kawaguchi-Ihara;I. Murohashi;N. Nara;S. Tohda
Wnt/β-连环蛋白信号传导参与各种类型癌细胞的生长。据报道，Wnt 3A促进造血干细胞的自我更新。材料与方法检测重组Wnt 3A蛋白对4种急性髓系白血病（AML）和4种急性T淋巴细胞白血病（T-ALL）细胞系体外生长的影响。结果Wnt 3A刺激对这些细胞系的短期生长没有影响或轻微抑制。在三个细胞系中，Wnt 3A促进悬浮培养后的克隆形成细胞的恢复，表明促进白血病干细胞或祖细胞的自我更新能力。免疫印迹分析显示，Wnt 3A刺激减少了这些细胞中磷酸化的β-连环蛋白，并增加了β-连环蛋白，表明Wnt 3A刺激激活了Wnt/β-连环蛋白信号传导。结论Wnt 3A刺激不能促进整个细胞群的生长，但能促进某些AML和T-ALL细胞系中白血病干/祖细胞的自我更新。
BACKGROUND Wnt/beta-catenin signaling is involved in the growth of various types of cancer cells. Wnt3A has been reported to promote the self-renewal of hematopoietic stem cells. MATERIALS AND METHODS The effects of recombinant Wnt3A protein on the in vitro growth of four acute myeloid leukemia (AML) and four acute T-lymphoblastic leukemia (T-ALL) cell lines was examined. RESULTS Wnt3A stimulation either had no effect on, or slightly suppressed, the short-term growth of these cell lines. In three cell lines, Wnt3A promoted clonogenic cell recovery after suspension culture, suggesting the promotion of the self-renewal capacity of leukemic stem or progenitor cells. Immunoblot analysis showed that Wnt3A stimulation reduced phosphorylated beta-catenin and increased beta-catenin in these cells, indicating that Wnt3A stimulation activated Wnt/beta-catenin signaling. CONCLUSION Wnt3A stimulation did not promote the growth of whole cell populations, but did promote the self-renewal of leukemic stem/progenitor cells in some AML and T-ALL cell lines.