Phylogenetic and transcriptional analysis of chrysanthemum GRAS transcription factors
Phylogenetic and transcriptional analysis of chrysanthemum GRAS transcription factors
复制标题
菊花GRAS转录因子的系统发育和转录分析
DOI：
10.1007/s10535-018-0816-1
复制
发表时间：
2018-12-01
影响因子：
1.5
通讯作者：
Chen, S. M.
中科院分区：
文献类型：
--
作者：
Gao, T. W.;Zhang, W. W.;Chen, S. M.
GRAS转录因子编码的蛋白质在400至700个残基之间，由其保守的C端识别。在这里，一组23 CmGRAS基因被确定从扫描的菊花（Chrysanthemum morifolium）转录组。系统发育分析表明，这些基因中有9个可以被分配到GRAS基因家族的直系同源物，其中4个形成了两对旁系同源物。基于菊花的GRAS蛋白家族的系统发育分析和最近对8种代表性被子植物的研究表明，大多数CmGRAS基因属于一个公认的亚组。CmGRAS 4和CmGRAS 10分别在花和根中强烈转录。CmGRAS 19和CmGRAS 20的DELLA亚家族转录本丰度在生殖组织中很高，并且它们对植物激素和胁迫有响应。建立已知的代表性GRAS基因与CmGRAS之间的同源关系，以及CmGRAS基因在植物激素处理或胁迫后的转录谱，将有助于后续GRAS基因家族的功能分析。
The GRAS transcription factors encoding proteins ranging from 400 to 700 residues are recognized by their conserved C terminus. Here, a set of 23 CmGRAS genes was identified from a scan of the chrysanthemum (Chrysanthemum morifolium) transcriptome. A phylogenetic analysis implied that nine of these genes could be assigned orthologs to the GRAS gene family, and that four of them formed two pairs of paralogs. A phylogenetic analysis of the GRAS protein family based on the chrysanthemum and recent study of eight representative species of angiosperms showed that most of the CmGRAS genes belong to a recognized sub-group. CmGRAS4 and CmGRAS10 were strongly transcribed in flowers and roots, respectively. The DELLA subfamily transcript abundance of the CmGRAS19 and CmGRAS20 was high in the reproductive tissues, and they were responsive to phytohormones and stresses. Establishment of the orthology relationships between the known representative GRAS genes and CmGRAS, and transcriptional profiles of CmGRASs after phytohormone treatments or stresses will facilitate subsequent functional analyses in the GRAS gene family.