Forster resonance energy transfer-FRET what is it, why do it, and how it's done
Forster resonance energy transfer-FRET what is it, why do it, and how it's done
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DOI：
10.1016/s0075-7535(08)00001-6
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Clegg, Robert M.
中科院分区：
文献类型：
--
作者：
Clegg, Robert M.
在过去的25年中，福斯特共振能量转移（FRET）技术的应用得到了极大的扩展，该技术已成为许多生物和生物物理领域的主要技术。许多出版物每周都使用FRET和大多数应用使用FRET作为光谱研究工具。在本章中，我们通过强调FRET途径与所有其他去激励途径的动力学竞争，研究了共振能量传递的一些一般显著特征。这种方法强调了FRET的许多生物技术和生物物理用途，以及强调了FRET在光合作用中的重要竞争过程和生物学功能。
The applications of Forster resonance energy transfer (FRET) have expanded tremendously in the last 25 years, and the technique has become a staple technique in many biological and biophysical fields. Many publications appear weekly using FRET and most of the applications use FRET as a spectroscopic research tool. In this chapter, we have examined some general salient features of resonance energy transfer by stressing the kinetic competition of the FRET pathway with all other pathways of de-excitation. This approach emphasizes many of the biotechnological and biophysical uses of FRET, as well as emphasizing the important competing processes and biological functions of FRET in photosynthesis.