Side population cells expressing ABCG2 in human adult dental pulp tissue
Side population cells expressing ABCG2 in human adult dental pulp tissue
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DOI：
10.1111/j.1365-2591.2007.01301.x
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发表时间：
2007-12-01
影响因子：
5
通讯作者：
Ueda, M.
中科院分区：
文献类型：
--
作者：
Honda, M. J.;Nakashima, F.;Ueda, M.
目的应用Hoechst排除法检测人牙髓组织中SP细胞的存在情况。用Hoechst 33342对细胞进行染色，并分选为SP细胞或非SP细胞[主群（MP）细胞]。结果SP细胞表达ABCG 2、Nestin、Notch-1和α-平滑肌肌动蛋白的频率为0.67%~ 1.02%。维拉帕米可使SP谱消失。结论成人牙髓组织中含有SP细胞，SP细胞可分化为成牙本质细胞样细胞。
Aim To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.Methodology Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.Results SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disapeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured osteogenic medium.Conclusion Human adult dental pulp tissue contains SP cells that diferentiate into odontoblast-like cells.