Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa
Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa
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DOI：
10.1111/j.1574-6968.2005.00024.x
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Isono, K
中科院分区：
文献类型：
--
作者：
Gan, X;Arita, K;Isono, K
线粒体核糖体（线粒体核糖体）从其假定的原核生物祖先高度进化而来，并且在不同生物体之间差异很大。为了进一步了解其结构和进化特征，我们用质谱法纯化和鉴定了粗神经孢子菌的线粒体核糖体蛋白，并将其与出芽酵母酿酒酵母进行了比较。两种真菌的线粒体核糖体蛋白大部分相互保守性较好，尽管保守程度有很大差异。其中一种线粒体核糖体蛋白与酵母Mhrlp同源，参与酵母线粒体同源DNA重组和基因组维持。
The mitochondrial ribosome (mitoribosome) has highly evolved from its putative prokaryotic ancestor and varies considerably from one organism to another. To gain further insights into its structural and evolutionary characteristics, we have purified and identified individual mitochondrial ribosomal proteins of Neurospora crassa by mass spectrometry and compared them with those of the budding yeast Saccharomyces cerevisiae. Most of the mitochondrial ribosomal proteins of the two, fungi are well conserved with each other, although the degree of conservation varies to a large extent. One of the N. crassa mitochondrial ribosomal proteins was found to be homologous to yeast Mhrlp that is involved in homologous DNA recombination and genome maintenance in yeast mitochondria.