Inhibitory activity of nm23-H1 on invasion and colonization of human prostate carcinoma cells is not mediated by its NDP kinase activity
Inhibitory activity of nm23-H1 on invasion and colonization of human prostate carcinoma cells is not mediated by its NDP kinase activity
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DOI：
10.1016/s0304-3835(99)00236-0
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发表时间：
1999-10-18
期刊：
影响因子：
9.7
通讯作者：
Lee, H
中科院分区：
文献类型：
--
作者：
Lee, HY;Lee, H
人nm 23-H1基因产物，一种假定的转移抑制因子。被鉴定为核苷二磷酸激酶（NDPK）A亚型。为了研究nm 23-H1的NDPK活性对其抑制转移表型组分的功能效应，我们用编码nm 23-H1突变蛋白的cDNA转染人前列腺癌细胞系DU 145，该突变蛋白缺乏NDPK活性。与对照组相比，突变型nm 23-H1转染细胞系在软琼脂中的侵袭力和定殖率均降低，而野生型nm 23-H1转染细胞系则无此表现。提示nm 23-H1的转移抑制作用不依赖于NDPK酶活性。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
The human nm23-H1 gene product, a putative metastasis suppressor. was identified as nucleoside diphosphate kinase (NDPK) A isoform. To investigate the functional effect of nm23-H1's NDPK activity on its suppression of the components of metastatic phenotype, we transfected a human prostate carcinoma cell line, DU145, with the cDNA encoding nm23-H1 mutant protein lacking NDPK activity. The mutant nm23-H1 transfected cell lines displayed decreased invasiveness and colonization in soft agar as the wild-type nm23-H1 transfectants did when compared with the control transfected line. The results suggest that the metastasis suppressing function of nm23-H1 is independent of the NDPK enzymatic activity. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.