Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells
Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells
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DOI：
10.1016/j.tvjl.2013.10.033
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发表时间：
2014-01-01
影响因子：
2.2
通讯作者：
Matsumoto, Taro
中科院分区：
文献类型：
--
作者：
Kono, Shota;Kazama, Tomohiko;Matsumoto, Taro
据报道，成熟脂肪细胞来源的去分化脂肪（DFAT）细胞显示出与间充质干细胞相似的多向分化潜能。由于DFAT细胞可以从少量脂肪组织中制备，因此它们可以促进猫等小型伴侣动物的细胞治疗。本研究探讨了是否可以从猫脂肪组织中产生多能DFAT细胞，并将DFAT细胞的特性与脂肪源性干细胞（ASCs）的特性进行了比较。DFAT细胞和ASC分别从胶原酶消化的猫网膜脂肪组织的漂浮成熟脂肪细胞部分和基质血管部分制备。两种细胞类型的生长动力学，集落形成单位成纤维细胞（CFU-F）的频率，免疫表型特性，和多谱系分化potential.DFAT细胞和ASCs可以产生约1克的脂肪组织和生长和传代培养层粘连蛋白包被的盘子。第1代DFAT细胞CFU-Fs频率（35.8%）显著高于ASCs（20.8%）（P1）。DFAT细胞和ASC具有相似的免疫表型（CD 44（+）、CD 90（+）、CD 105（+）、CD 14（-）、CD 34（-）和CD 45（-））。在P1时，在ASC中容易检测到α-平滑肌肌动蛋白阳性细胞（15.2 +/- 7.2%），但在DFAT细胞中很少检测到α-平滑肌肌动蛋白阳性细胞（2.2 +/- 3.2%）。两种细胞类型在体外均表现出成脂、成骨、成软骨和平滑肌细胞分化潜能。总之，猫DFAT细胞表现出与ASC相似的特性，但显示出更高的CPU-F频率和更大的同质性。DFAT细胞，如ASCs，可能是一个有吸引力的来源，为基于细胞的治疗猫。(C)2013爱思唯尔有限公司版权所有。
It has been reported that mature adipocyte-derived dedifferentiated fat (DFAT) cells show multilineage differentiation potential similar to that observed in mesenchymal stem cells. Since DFAT cells can be prepared from a small quantity of adipose tissue, they could facilitate cell-based therapies in small companion animals such as cats. The present study examined whether multipotent DFAT cells can be generated from feline adipose tissue, and the properties of DFAT cells were compared with those of adipose-derived stem cells (ASCs). DFAT cells and ASCs were prepared from the floating mature adipocyte fraction and the stromal vascular fraction, respectively, of collagenase-digested feline omental adipose tissue. Both cell types were evaluated for growth kinetics, colony-forming unit fibroblast (CFU-F) frequency, immunophenotypic properties, and multilineage differentiation potential.DFAT cells and ASCs could be generated from approximately 1 g of adipose tissue and were grown and subcultured on laminin-coated dishes. The frequency of CFU-Fs in DFAT cells (35.8%) was significantly higher than that in ASCs (20.8%) at passage 1 (P1). DFAT cells and ASCs displayed similar immunophenotypes (CD44(+), CD90(+), CD105(+), CD14(-), CD34(-) and CD45(-)). Alpha-smooth muscle actin-positive cells were readily detected in ASCs (15.2 +/- 7.2%) but were rare in DFAT cells (2.2 +/- 3.2%) at P1. Both cell types exhibited adipogenic, osteogenic, chondrogenic, and smooth muscle cell differentiation potential in vitro. In conclusion, feline DFAT cells exhibited similar properties to ASCs but displayed higher CPU-F frequency and greater homogeneity. DFAT cells, like ASCs, may be an attractive source for cell-based therapies in cats. (C) 2013 Elsevier Ltd. All rights reserved.