Reversed potentials in isolated frog skin. II. Active transport of chloride
Reversed potentials in isolated frog skin. II. Active transport of chloride
复制标题
离体青蛙皮肤的反转电位。
DOI：
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发表时间：
1966
影响因子：
5.6
通讯作者：
P. Curran
中科院分区：
文献类型：
--
作者：
D. Martin;P. Curran
在没有电化学电位差的情况下，在两种青蛙的皮肤中证实了Cl的净向内运输。pipiens和R.在低（2 mM）Cl浓度的条件下，在洗浴溶液中的esculenta。研究了红腹锦鸡儿皮肤的电位分布。在内部溶液相对于外部溶液为负的条件下，用微电极检测pipiens。正常电位差的这种反转被发现是由于皮肤中面向外的电屏障上的电位变化而引起的。反向的电位差出现，至少在一定程度上，作为向内的Cl运输的结果。这种效应可能是由于产电Cl转移或Cl运输引起的上皮细胞内部组成的变化。
Net inward transport of Cl in the absence of an electrochemical potential difference was demonstrated in the skin of two species of frog, R. pipiens and R. esculenta under conditions of low (2 mM) Cl concentration in the bathing solutions. The electrical potential profile of skins of R. pipiens was examined with microelectrodes under conditions in which the inside solution was negative relative to the outside solution. This reversal of the normal potential difference was found to arise as a result of potential changes across the outward facing electrical barriers in the skin. The reversed potential difference appears to arise, at least in part, as a result of the inward Cl transport. The effect may be due either to electrogenic Cl transfer or to variations in internal composition of the epithelial cells arising as a result of Cl transport.