On the Interaction of Cold Atmospheric Pressure Plasma with Surfaces of Bio-molecules and Model Polymers
On the Interaction of Cold Atmospheric Pressure Plasma with Surfaces of Bio-molecules and Model Polymers
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DOI：
10.1007/s11090-015-9673-2
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发表时间：
2016-01-01
影响因子：
3.6
通讯作者：
Oehrlein, G. S.
中科院分区：
文献类型：
--
作者：
Bartis, E. A. J.;Knoll, A. J.;Oehrlein, G. S.
本文综述了国内外在表面微放电（SMD）和常压等离子体射流（APPJ）与模型聚合物和生物分子表面相互作用机制方面的研究进展。我们讨论了等离子体源类型、操作参数和气体环境对表面修饰和生物失活的影响。我们关注的是温和的、遥远的条件，在那里可见的等离子体羽流不接触表面。对于以Ar为燃料的APPJ，等离子体羽流与O-2和/或N-2气体环境的相互作用导致氧化和表面结合的NOx，即使在不含氧和氮的材料上也是如此。APPJ还可以修饰光敏聚合物。使用滤光片，这些修饰部分是由于在光谱范围内与Ar准分子发射相对应的真空紫外（VUV）光子照射造成的。以O-2/N-2操作的SMD源未见vuv诱导效应。使用O-2/N-2混合物进行SMD处理会导致表面氧化和氮化。在聚合物和生物分子上发现了一种新的表面结合物质NO3。根据气体化学性质和膜分子结构的不同，NO3表面浓度可达10%。通过酶联免疫吸附试验评估，血浆处理过的脂多糖（LPS）膜表面NO3（一种存在于大肠杆菌等细菌中的免疫刺激生物分子）和整体表面氧化与LPS生物失活相关。使用SMD研究了环境湿度，发现在富含o -2的条件下，环境湿度会降低整体表面修饰，包括NO3和生物失活。最后，我们讨论了可能的机制，并将我们的结果与已发表的模拟研究进行了比较。
We review studies of surface-interaction mechanisms for a surface microdis-charge (SMD) and an atmospheric pressure plasma jet (APPJ) with model polymers and biomolecules in our laboratory. We discuss the influence of plasma source type, operating parameters, and gaseous environments on surface modifications and biological deactivation. We focus on mild, remote conditions where the visible plasma plume does not contact the surface. For an APPJ fed with Ar, the interaction of the plasma plume with O-2 and/or N-2 gaseous environments leads to oxidation and surface-bound NOx even on materials containing neither oxygen nor nitrogen. The APPJ also modifies photo-sensitive polymers. Using optical filters, these modifications were shown to result in part from irradiation with vacuum ultraviolet (VUV) photons in a spectral range corresponding to Ar excimer emission. No VUV-induced effects were seen for the SMD source operated with O-2/N-2. SMD treatments using O-2/N-2 mixtures result in surface oxidation and nitridation. A new surface-bound species, NO3, has been measured on the polymers and biomolecules. Depending on the gas chemistry and film molecular structure, the NO3 surface concentration can reach 10 %. Both surface NO3 on plasma-treated films of lipopolysaccharide (LPS), an immune stimulating biomolecule found in bacteria such as E. coli, and overall surface oxidation correlate with LPS biological deactivation as evaluated using an enzyme-linked immunosorbent assay. Ambient humidity was studied using the SMD and was found to decrease overall surface modifications including NO3 and biodeactivation for O-2-rich conditions. Lastly, we discuss possible mechanisms and compare our results with published simulation studies.