Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane
Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane
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DOI：
10.1007/s00438-010-0546-3
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发表时间：
2010-07-01
影响因子：
3.1
通讯作者：
D'Hont, Angelique
中科院分区：
文献类型：
--
作者：
Piperidis, George;Piperidis, Nathalie;D'Hont, Angelique
现代甘蔗栽培品种（甘蔗属，2n = 100-120）是由世纪前产糖种S. officinarum L.野生种S. [医]手打木利用基因组原位杂交技术，我们发现现代栽培品种的基因组中有15%到27.5%来自于S。spontaneum，包括10-23%来自该野生种的整个染色体和8-13%来自种间重组的染色体。我们证实了2n + n传递在这两个物种之间的杂交和第一次回交中的发生，并证明了这也可以发生在S.药用植物和现代栽培品种我们分析了五个S。officinarum克隆的染色体数目超过80条，证明它们是种间杂交的结果，支持了该物种2n = 80的经典观点。我们还说明了分子细胞遗传学和遗传作图方法分析复杂的基因组之间的互补性。
Modern sugarcane cultivars (Saccharum spp., 2n = 100-120) are complex polyploids derived from interspecific hybridization performed a century ago between the sugar-producing species S. officinarum L. and the wild species S. spontaneum L. Using genomic in situ hybridization, we revealed that between 15 and 27.5% of the genome of modern cultivars is derived from S. spontaneum, including 10-23% of entire chromosomes from this wild species and 8-13% chromosomes derived from interspecific recombination. We confirmed the occurrence of 2n + n transmission in crosses and first backcrosses between these two species and demonstrated that this also can occur in crosses between S. officinarum and modern cultivars. We analysed five S. officinarum clones with more than 80 chromosomes and demonstrated that they were derived from interspecific hybridization supporting the classical view that this species is characterized by 2n = 80. We also illustrated the complementarities between molecular cytogenetics and genetic mapping approaches for analysing complex genomes.