Neurogenesis enhances response specificity to spatial pattern stimulation in hippocampal cultures
Neurogenesis enhances response specificity to spatial pattern stimulation in hippocampal cultures
复制标题
神经发生增强海马培养物对空间模式刺激的反应特异性
DOI：
10.1109/tbme.2016.2639468
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Jimbo Y.
中科院分区：
文献类型：
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作者：
Tanaka Y;Isomura T;Shimba K;Kotani K;Jimbo Y.
海马中的成年神经发生促进了哺乳动物的认知功能，如模式分离。然而，目前尚不清楚新生神经元如何介导神经网络的变化，以提高模式分离能力。在这里，我们开发了一个体外模型，成人神经发生使用大鼠海马培养，以调查是否新生神经元可以直接纳入神经网络相关的模式分离，以产生功能improvements.MethodWe优化的时间表，碱性成纤维细胞生长因子（bFGF）的管理，以提高神经发生，然后使用微电极阵列系统来评估神经培养物对两种不同空间模式刺激的反应（L和倒L形）之前和之后的training.ResultsWe发现，早期突触反应时间到一个给定的模式训练后缩短，这种效果是更明显的bFGF处理的文化。此外，bFGF处理的培养物显示出改善的反应特异性训练后所示的计算Kullback-Leibler分歧值，这表明模式分离更好地实现了文化与增强neurogenes.ConclusionNeural网络包含更大数量的未成熟神经元表现出更高的反应特异性空间模式的刺激，提示通过神经发生增强来改善模式分离。重要性这些结果是第一次在体外证明神经发生改善模式分离。我们的新的体外系统将是一个有用的工具，调查成人神经发生的认知功能的贡献。
ObjectiveAdult neurogenesis in the hippocampus facilitates cognitive functions such as pattern separation in mammals. However, it remains unclear how newborn neurons mediate changes in neural networks to enhance the pattern separation ability. Here, we developed an in vitro model of adult neurogenesis using rat hippocampal cultures in order to investigate whether newborn neurons can be directly incorporated into neural networks related to pattern separation to produce functional improvements.MethodWe optimized at schedule of basic fibroblast growth factor (bFGF) administration to enhance neurogenesis, and then used a microelectrode array system to evaluate the responses of neural cultures to two different spatial pattern stimuli (L and inverted L shapes) before and after training.ResultsWe found that early synaptic response times to a given pattern were shortened after training, and that this effect was more pronounced in cultures treated with bFGF. Furthermore, bFGF-treated cultures showed improved response specificity after training as indicated by calculated Kullback-Leibler divergence values, suggesting that pattern separation was better achieved in cultures with enhanced neurogenesis.ConclusionNeural networks containing greater numbers of immature neurons exhibited higher response specificity to spatial pattern stimulation, suggesting the improvement of the pattern separation by neurogenesis enhancement.SignificanceThese results are the first in vitro demonstration that neurogenesis improves pattern separation. Our novel in vitro system will be a useful tool for investigating the contribution of adult neurogenesis to cognitive functions.