A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant
A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant
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DOI：
10.1093/jxb/erh044
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发表时间：
2004-02-01
影响因子：
6.9
通讯作者：
Shimazaki, K
中科院分区：
文献类型：
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作者：
Doi, M;Shigenaga, A;Shimazaki, K
向光蛋白（phot1和phot2）被认为是多功能的蓝光（BL）受体，介导向光性、叶绿体运动、气孔开放和叶片扩展。光1光2双突变体缺乏所有这些反应。为了证实BL反应中对光致蛋白的需求，用PHOT1 cDNA转化拟南芥phot1 phot2双突变体，并在转化体中分析表型恢复。结果发现，所有的BL响应恢复，虽然差异，转化的拟南芥phot1 phot2双突变体与PHOT1 cDNA。结果表明，phot1是植物所有这些BL反应的重要组成部分，细胞水平的phot1可能决定个体BL反应。
Phototropins (phot1 and phot2) are suggested to be multifunctional blue-light (BL) receptors mediating phototropism, chloroplast movement, stomatal opening, and leaf expansion. The Arabidpsis phot1 phot2 double mutant lacks all of these responses. To confirm the requirement of phototropins in BL responses, the Arabidopsis phot1 phot2 double mutant was transformed with PHOT1 cDNA and the phenotypic restoration was analysed in the transformants. It was found that all BL responses were restored, although differentially, by the transformation of the Arabidopsis phot1 phot2 double mutant with PHOT1 cDNA. The results showed that phot1 was an essential component for all these BL responses in planta, and that the cellular level of phot1 might determine the individual BL responses.