Localized chemical stimulation of cellular micropatterns using a porous membrane-based culture substrate
Localized chemical stimulation of cellular micropatterns using a porous membrane-based culture substrate
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DOI：
10.1016/j.snb.2004.12.090
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发表时间：
2005-07
影响因子：
8.4
通讯作者：
K. Takoh;Takeshi Ishibashi;T. Matsue;M. Nishizawa
中科院分区：
文献类型：
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作者：
K. Takoh;Takeshi Ishibashi;T. Matsue;M. Nishizawa
我们已经研究了一种技术，用于将化学品局部递送到蜂窝网络的一部分。通过结合由多孔聚碳酸酯膜制成的可渗透基底和由具有图案化孔（直径，50-200μm）的弹性体聚二甲基硅氧烷（PDMS）膜制成的微掩模来制备用于局部刺激的装置。作为细胞网络的模型，将原代心肌细胞在膜上培养为单层片和线图案，然后通过微掩模中的孔将辛醇（间隙连接抑制剂）从多孔基底的相反相局部递送至细胞。这些细胞的细胞间通讯进行了评价，通过观察胞质Ca 2+瞬变。在肌细胞片上的同步Ca 2+转换仅在由微掩模限定的区域处停止。基于使用线图案化的心肌细胞的实验，证明了电缀合的细胞网络被跨细胞网络的辛醇刺激分成独立的部分。本研究的目的是开发一种基于细胞网络的生物传感系统。
We have investigated a technique for the local delivery of chemicals to part of a cellular network. The device for the local stimulation was prepared by binding a permeable substrate made of a porous polycarbonate membrane and a micromask made of an elastomeric poly-(dimethylsiloxane) (PDMS) film having a patterned hole (diameter, 50–200μm). As the model of the cellular network, primary cardiac myocytes were cultured on the membrane as a monolayer sheet and a line pattern, and then octanol (a gap junctional inhibitor) was locally delivered to the cells from the opposite phase of the porous substrate through a hole in the micromask. Intercellular communications of these cells were evaluated by observing the cytosolic Ca2+transients. The synchronous Ca2+transition on a myocyte sheet was stopped at only the area defined by the micromask. Based on the experiments using the line-patterned myocytes, it was proved that the electrically conjugating cellular network was divided into independent parts by the octanol stimulation across the cellular network. This research was carried out with the objective of developing a biosensing system based on a cellular network.