Functionalization of lactose as a biological carrier for bovine serum albumin by electrospraying
Functionalization of lactose as a biological carrier for bovine serum albumin by electrospraying
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DOI：
10.1016/j.ijpharm.2011.04.045
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发表时间：
2011-07-29
影响因子：
5.8
通讯作者：
van Ommen, J. R.
中科院分区：
文献类型：
--
作者：
Cardoso, M. A. Tavares;Talebi, M.;van Ommen, J. R.
电流体动力学雾化（EHDA）是一种制备新型药物复合微粒的有吸引力的技术。这项工作的目的是证明EHDA可以成功地用于将蛋白质的纳米/微米颗粒附着到乳糖上，乳糖是用于肺部递送的常用赋形剂，在复合物溶解后保持蛋白质的所有生物学特性。牛血清白蛋白（BSA）被用作模型蛋白。用二甲基亚砜（DMSO）和乙醇两种不同的溶剂对BSA的雾化进行了测试。使用DMSO的方法导致形成蛋白质薄层，而使用乙醇的测试导致形成平均直径约为700 nm的球形颗粒。以乙醇为溶剂，通过静电力将BSA吸附在乳糖表面，形成复合物。未观察到蛋白质的变性或显著构象变化，尽管乳糖暴露于溶液射流的增加降低了方法的重现性。由于模型蛋白质中不存在变性，因此可以测试这种新方法用于生产药物递送系统的干粉的新制剂。(C)2011 Elsevier B. V.保留所有权利。
Electrohydrodynamic atomization (EHDA) is an attractive technique to make new types of composite particles for pharmaceutical use. The aim of this work is to prove that EHDA can be successfully used to attach nano/micro-particles of protein to lactose, the commonly used excipient for pulmonary delivery, keeping all the biological properties of the protein after dissolution of the complex. Bovine serum albumin (BSA) was used as a model protein. The atomization of BSA was tested with two different solvents, dimethyl sulfoxide (DMSO) and ethanol. The process using DMSO resulted in the formation of a thin layer of protein while the tests using ethanol resulted in the formation of spherical particles with mean diameters around 700 nm. Ethanol as solvent was also used to produce a composite formed by BSA adsorbed at the surface of lactose by electrostatic forces. No denaturation or significant conformational changes of the protein were observed, although an increase in the exposition of the lactose to the jet of the solution decreases the reproducibility of the method. Due to the absence of denaturation in the model protein, this new approach can be tested for the production of new formulations for dry powders for drug delivery systems. (C) 2011 Elsevier B.V. All rights reserved.