Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B
Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B
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两种昆虫抗菌肽 Moricin 和 Cecropin B 对猪细菌病原体具有广泛的活性
DOI：
10.1007/s10059-013-2132-0
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发表时间：
2013-02-01
影响因子：
3.8
通讯作者：
He, Qigai
中科院分区：
文献类型：
--
作者：
Hu, Han;Wang, Chunmei;He, Qigai
作为对感染的反应，昆虫产生各种抗菌肽(AMPs)来杀死入侵的病原体。为了研究它们的理化性质及其在养猪业中的临床和商业应用，我们用化学方法合成了成熟肽桑蚕素和透明颤菌抗菌肽B。抗菌素对8株供试菌株的最小抑菌浓度(MIC)为8~12 8μg/ml，抗菌素B对革兰氏阴性菌的最低抑菌浓度(MICs)为0.5~16μg/ml，与对大多数致病菌表现出的较强的抗菌活性相比，它们对猪红细胞的溶血活性有限。进一步研究了Moricin和Cecropin B对副猪嗜血杆菌SH 0165的抑制作用。用Moricin和Cecropin B处理H.ParasuisSH 0165表明对细菌的细胞膜有广泛的损伤。对Moricin和Cecropin B用来消除病原体的可能机制也提出了见解。本研究的观察结果对AMPS在养猪业中的应用具有重要意义。
In response to infection, insects produce a variety of antimicrobial peptides (AMPs) to kill the invading pathogens. To study their physicochemical properties and bioactivities for clinical and commercial use in the porcine industry, we chemically synthesized the mature peptidesBombyx morimoricin andHyalophora cecropiacecropin B. In this paper, we described the antimicrobial activity of the two AMPs. Moricin exhibited antimicrobial activity on eight strains tested with minimal inhibitory concentration values (MICs) ranging between 8 and 128 μg/ml, while cecropin B mainly showed antimicrobial activity against the Gramnegative strains with MICs ranging from 0.5 to 16 μg/ml. Compared to the potent antimicrobial activity these two AMPs displayed against most of the bacterial pathogens tested, they exhibited limited hemolytic activity against porcine red blood cells. The activities of moricin and cecropin B againstHaemophilus parasuisSH 0165 were studied in further detail. Transmission electron microscopy (TEM) of moricin and cecropin B treatedH. parasuisSH 0165 indicated extensive damage to the membranes of the bacteria. Insights into the probable mechanism utilized by moricin and cecropin B to eliminate pathogens are also presented. The observations from this study are important for the future application of AMPs in the porcine industry.