Trityl Quinodimethane Derivatives as Unimolecular Triple-Function Extracellular EPR Probes for Redox, pH, and Oxygen
Trityl Quinodimethane Derivatives as Unimolecular Triple-Function Extracellular EPR Probes for Redox, pH, and Oxygen
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DOI：
10.1021/acs.analchem.2c03754
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发表时间：
2023-01-05
影响因子：
7.4
通讯作者：
Liu，Yangping
中科院分区：
文献类型：
--
作者：
Feng，Yalan;Tan，Xiaoli;Liu，Yangping
电子顺磁共振（EPR）光谱和成像结合使用合适的探针是一个有前途的工具，用于评估肿瘤微环境（TME）。通过EPR测量多个TME参数是非常可取的，但具有挑战性。在此，我们设计并合成了一类带负电荷的三苯甲基醌二甲烷MTPs作为单分子三功能细胞外探针的氧化还原，pH值和氧气（O2）水平。使用氘代类似物，dMTP 5，它具有最佳的pKaas和高灵敏度的生物还原和O2，我们合理地评估pH值的影响，从HepG2细胞和细胞O2消耗的还原剂流出。
Electron paramagnetic resonance (EPR) spectroscopy and imaging coupled with the use of suitable probes is a promising tool for assessment of the tumor microenvironment (TME). Measurement of multiple TME parameters by EPR is very desirable but challenging. Herein, we designed and synthesized a class of negative-charged trityl quinodimethane MTPs as unimolecular triple-function extracellular probes for redox, pH, and oxygen (O2) levels. Using the deuterated analogue, dMTP5,which has an optimal pKaas well as high sensitivity to bioreduction and O2, we reasonably evaluated pH effects on efflux of reducing agents from HepG2 cells and cellular O2consumption.