Genetic Analysis and Fine Mapping of Two Genes for Grain Shape and Weight in Rice
Genetic Analysis and Fine Mapping of Two Genes for Grain Shape and Weight in Rice
复制标题
水稻粒形和重量两个基因的遗传分析和精细定位。
DOI：
10.1111/j.1744-7909.2008.00793.x
复制
发表时间：
2009-01-01
影响因子：
11.4
通讯作者：
Qian, Qian
中科院分区：
文献类型：
--
作者：
Guo, Longbiao;Ma, Lilian;Qian, Qian
本研究以大粒籼稻品种保大利为材料，对控制粒重的遗传位点进行了鉴定。以其衍生的F（2）、F（3）和BC（2）F（2）为作图群体。连锁分析表明，两个控制粒重的基因（命名为GW3和GW6）分别定位在第3和第6染色体上。精细定位将GW3界定为两个序列标记位点标记（WGW 16和WGW 19）之间的122 kb物理距离，其含有由基因组研究所（http：//www.example.com）注释的16个开放阅读框。www.tigr.org GW6进一步定位在两个简单重复序列标记（RM7179和RM3187）之间。这些结果为水稻粒重的分子标记辅助选择和GW基因的进一步克隆奠定了基础，为进一步揭示水稻粒重的分子机理奠定了基础。
To identify genetic loci controlling grain weight, an elite indica rice variety, Baodali, with large grains was identified and used in this study. Its derived F(2), F(3) and BC(2)F(2) with another japonica rice variety Zhonghua 11 were used as mapping populations. Linkage analyses demonstrated that two genes controlling grain weight, designated as GW3 and GW6, were mapped to chromosome 3 and chromosome 6, respectively. Fine mapping delimited GW3 to a 122 kb physical distance between two sequence tagged site markers (WGW16 and WGW19) containing 16 open reading frames annotated by The Institute for Genomic Research (http://www.tigr.org). GW6 was further mapped between two simple sequence repeat markers (RM7179 and RM3187). These results are useful for both marker assisted selection of grain weight, and for further cloning of GW genes, which will contribute to the dissection of the molecular mechanism underlying grain weight in rice.