Food additives such as sodium sulphite, sodium benzoate and curcumin inhibit leptin release in lipopolysaccharide-treated murine adipocytes in vitro
Food additives such as sodium sulphite, sodium benzoate and curcumin inhibit leptin release in lipopolysaccharide-treated murine adipocytes in vitro
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DOI：
10.1017/s0007114511003680
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发表时间：
2012-03-01
影响因子：
3.6
通讯作者：
Fuchs, Dietmar
中科院分区：
文献类型：
--
作者：
Ciardi, Christian;Jenny, Marcel;Fuchs, Dietmar
肥胖导致促炎通路的激活，导致低度炎症状态。最近，一些研究表明，暴露于脂多糖（LPS）可以启动并维持肥胖者的慢性低度炎症状态。随着食品添加剂的日摄入量大幅增加，本研究的目的是探讨食品添加剂对脂多糖存在下小鼠脂肪细胞中瘦素、IL-6和亚硝酸盐释放的潜在影响。测定小鼠3T3-L1脂肪细胞与LPS、食品防腐剂亚硫酸钠（SS）、苯甲酸钠（SB）、香料和着色剂姜黄素共孵育24 h后的上清液中瘦素、IL-6和亚硝酸盐的浓度，并分析瘦素分泌动力学。在与SB和姜黄素孵育12和24小时后，观察到瘦素浓度的显著下降和剂量依赖性，而SS在处理24小时后降低了瘦素浓度。此外，SS增加，姜黄素降低lps刺激的IL-6分泌，而SB无此作用。所研究的化合物都不影响亚硝酸盐的产生。食品添加剂SS、SB和姜黄素对体外培养脂肪细胞与LPS共孵育后瘦素释放的影响呈剂量依赖性和时间依赖性。在食用营养性食品添加剂过程中，瘦素释放减少可能会减少中枢神经系统接触到的循环瘦素的量，因此可能导致肥胖环境。
Obesity leads to the activation of pro-inflammatory pathways, resulting in a state of low-grade inflammation. Recently, several studies have shown that the exposure to lipopolysaccharide (LPS) could initiate and maintain a chronic state of low-grade inflammation in obese people. As the daily intake of food additives has increased substantially, the aim of the present study was to investigate a potential influence of food additives on the release of leptin, IL-6 and nitrite in the presence of LPS in murine adipocytes. Leptin, IL-6 and nitrite concentrations were analysed in the supernatants of murine 3T3-L1 adipocytes after co-incubation with LPS and the food preservatives, sodium sulphite (SS), sodium benzoate (SB) and the spice and colourant, curcumin, for 24 h. In addition, the kinetics of leptin secretion was analysed. A significant and dose-dependent decrease in leptin was observed after incubating the cells with SB and curcumin for 12 and 24 h, whereas SS decreased leptin concentrations after 24 h of treatment. Moreover, SS increased, while curcumin decreased LPS-stimulated secretion of IL-6, whereas SB had no such effect. None of the compounds that were investigated influenced nitrite production. The food additives SS, SB and curcumin affect the leptin release after co-incubation with LPS from cultured adipocytes in a dose- and time-dependent manner. Decreased leptin release during the consumption of nutrition-derived food additives could decrease the amount of circulating leptin to which the central nervous system is exposed and may therefore contribute to an obesogenic environment.