Strains of the soil fungus Mortierella show different degradation potentials for the phenylurea herbicide diuron
Strains of the soil fungus Mortierella show different degradation potentials for the phenylurea herbicide diuron
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DOI：
10.1007/s10532-013-9624-7
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发表时间：
2013-11-01
期刊：
影响因子：
3.6
通讯作者：
Rosendahl, Soren
中科院分区：
文献类型：
--
作者：
Ellegaard-Jensen, Lea;Aamand, Jens;Rosendahl, Soren
迄今为止，微生物农药降解研究主要集中在细菌上，尽管真菌也被证明可以降解农药。在这项研究中，我们澄清了背景的能力，常见的土壤真菌被孢霉降解苯脲类除草剂敌草隆。比较了5个被孢霉菌株对敌草隆的降解潜力，并探讨了碳、氮在降解过程中的作用。结果表明，不同被孢霉菌株对敌草隆的降解能力差异很大，降解敌草隆的菌株之间亲缘关系较近。敌草隆降解最快的碳和氮丰富的媒体，而次优的营养水平限制降解，使它不太可能被孢霉利用敌草隆作为碳源或氮源。降解动力学表明，敌草隆降解后形成代谢产物1-（3，4-二氯苯基）-3-甲基脲、1-（3，4-二氯苯基）脲和一种迄今未知的代谢产物1-（3，4-二氯苯基）-3-亚甲基脲。
Microbial pesticide degradation studies have until now mainly focused on bacteria, although fungi have also been shown to degrade pesticides. In this study we clarify the background for the ability of the common soil fungus Mortierella to degrade the phenylurea herbicide diuron. Diuron degradation potentials of five Mortierella strains were compared, and the role of carbon and nitrogen for the degradation process was investigated. Results showed that the ability to degrade diuron varied greatly among the Mortierella strains tested, and the strains able to degrade diuron were closely related. Degradation of diuron was fastest in carbon and nitrogen rich media while suboptimal nutrient levels restricted degradation, making it unlikely that Mortierella utilize diuron as carbon or nitrogen sources. Degradation kinetics showed that diuron degradation was followed by formation of the metabolites 1-(3,4-dichlorophenyl)-3-methylurea, 1-(3,4-dichlorophenyl)urea and an hitherto unknown metabolite suggested to be 1-(3,4-dichlorophenyl)-3-methylideneurea.