Peripheral Motor and Sensory Nerve Conduction following Transplantation of Undifferentiated Autologous Adipose Tissue-Derived Stem Cells in a Biodegradable US Food and Drug Administration-Approved Nerve Conduit
Peripheral Motor and Sensory Nerve Conduction following Transplantation of Undifferentiated Autologous Adipose Tissue-Derived Stem Cells in a Biodegradable US Food and Drug Administration-Approved Nerve Conduit
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DOI：
10.1097/prs.0000000000002291
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发表时间：
2016-07-01
影响因子：
3.6
通讯作者：
Prantl, Lukas
中科院分区：
文献类型：
--
作者：
Klein, Silvan M.;Vykoukal, Jody;Prantl, Lukas
背景：以雪旺细胞或干细胞分化为雪旺细胞的导管在神经缺损的神经再生中显示出良好的结果。方法：本试验的概念结合了通过市售神经引导导管和自体，未分化，脂肪组织来源的干细胞预植的神经修复。从大鼠身上获取脂肪组织来源的干细胞，随后将其植入美国食品和药物管理局批准的I型胶原蛋白导管中。建立10mm长的坐骨神经间隙，通过移植自体脂肪组织源性干细胞或脱细胞（对照组）导管进行神经修复。6个月后，评估运动神经和感觉神经传导速度。切除神经，通过苏木精和伊红、van Gieson染色和免疫组织化学（S100蛋白）染色检测轴突再生的质量。结果：脂肪组织源性干细胞处理的神经间隙表现出较好的神经再生能力，表现为更高的运动和感觉神经传导速度值。脂肪组织干细胞导管预处理组运动神经和感觉神经传导速度显著高于单纯导管处理组（p < 0.05）。脂肪组织源性干细胞组检测到S100免疫反应性增加。在这一组中，导管内的轴突排列更有组织。结论：脂肪组织源性干细胞移植可显著提高周围神经间隙内运动神经和感觉神经的传导速度。与单独的神经导管相比，预先播种导管内的轴突排列更有组织。这里使用的方法可以很容易地转化为临床治疗。
Background: Conduits preseeded with either Schwann cells or stem cells differentiated into Schwann cells demonstrated promising results for the outcome of nerve regeneration in nerve defects.Methods: The concept of this trial combines nerve repair by means of a commercially available nerve guidance conduit and preseeding with autologous, undifferentiated, adipose tissue-derived stem cells. Adipose tissue-derived stem cells were harvested from rats and subsequently seeded onto a U.S. Food and Drug Administration-approved type I collagen conduit. Sciatic nerve gaps 10 mm in length were created, and nerve repair was performed by the transplantation of either conduits preseeded with autologous adipose tissue-derived stem cells or acellular (control group) conduits. After 6 months, the motor and sensory nerve conduction velocity were assessed. Nerves were removed and examined by hematoxylin and eosin, van Gieson, and immunohistochemistry (S100 protein) staining for the quality of axonal regeneration.Results: Nerve gaps treated with adipose tissue-derived stem cells showed superior nerve regeneration, reflected by higher motor and sensory nerve conduction velocity values. The motor and sensory nerve conduction velocity were significantly greater in nerves treated with conduits preseeded with adipose tissue-derived stem cells than in nerves treated with conduits alone (p < 0.05). Increased S100 immunoreactivity was detected for the adipose tissue-derived stem cell group. In this group, axon arrangement inside the conduits was more organized.Conclusions: Transplantation of adipose tissue-derived stem cells significantly improves motor and sensory nerve conduction velocity in peripheral nerve gaps. Preseeded conduits showed a more organized axon arrangement inside the conduit in comparison with nerve conduits alone. The approach used here could readily be translated into a clinical therapy.