Single cell transcriptomics of the developing zebrafish lens and identification of putative controllers of lens development.
Single cell transcriptomics of the developing zebrafish lens and identification of putative controllers of lens development.
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DOI：
10.1016/j.exer.2021.108535
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发表时间：
2021-05
影响因子：
3.4
通讯作者：
Miller AC
中科院分区：
文献类型：
--
作者：
Farnsworth DR;Posner M;Miller AC
脊椎动物晶状体是一个有价值的模型系统，用于研究协调组织分化的基因表达变化，因为它包含两种空间上分离的细胞类型，即它们支持的外上皮细胞和更深的去核纤维细胞。斑马鱼是研究晶状体发育的有用模型系统，因为该器官在生命的最初几天内在可接近的透明胚胎中快速发育。虽然我们对各种晶状体晶状体蛋白和控制晶状体发育的基本基因调控网络有深入的基础知识，但没有研究以单细胞分辨率详细描述脊椎动物晶状体中的基因表达。在这里，我们报告了斑马鱼胚胎和幼虫在单细胞分辨率下五天发育期间晶状体基因表达的图谱，确定了一些新的假定的晶状体发育调节因子。我们的数据解决了晶状体发育过程中α-晶状体蛋白的时空表达的开放性问题，这将支持未来对其功能的研究，并提供晶状体内外β-和γ-晶状体蛋白表达的第一个详细视图。我们描述了斑马鱼中作为旁系同源对出现的转录因子基因的不同表达。最后，我们检查了细胞骨架、膜相关、RNA 结合和转录因子基因的表达动态，识别出许多新模式。总的来说，这些数据为识别和表征晶状体发育调节机制提供了基础，并揭示了具有潜在治疗影响的未来功能研究的目标。
The vertebrate lens is a valuable model system for investigating the gene expression changes that coordinate tissue differentiation due to its inclusion of two spatially separated cell types, the outer epithelial cells and the deeper denucleated fiber cells that they support. Zebrafish are a useful model system for studying lens development given the organ’s rapid development in the first several days of life in an accessible, transparent embryo. While we have strong foundational knowledge of the diverse lens crystallin proteins and the basic gene regulatory networks controlling lens development, no study has detailed gene expression in a vertebrate lens at single cell resolution. Here we report an atlas of lens gene expression in zebrafish embryos and larvae at single cell resolution through five days of development, identifying a number of novel putative regulators of lens development. Our data address open questions about the temperospatial expression of α-crystallins during lens development that will support future studies of their function and provide the first detailed view of β- and γ-crystallin expression in and outside the lens. We describe divergent expression in transcription factor genes that occur as paralog pairs in the zebrafish. Finally, we examine the expression dynamics of cytoskeletal, membrane associated, RNA-binding, and transcription factors genes, identifying a number of novel patterns. Overall these data provide a foundation for identifying and characterizing lens developmental regulatory mechanisms and revealing targets for future functional studies with potential therapeutic impact.
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影响因子：
4.5
作者：
Bertrand S;Thisse B;Tavares R;Sachs L;Chaumot A;Bardet PL;Escrivà H;Duffraisse M;Marchand O;Safi R;Thisse C;Laudet V
通讯作者：
Laudet V
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4.3
作者：
Eden E;Lipson D;Yogev S;Yakhini Z
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Yakhini Z
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作者：
Cheng, SH;Shakespeare, T;Valdimarsson, G
通讯作者：
Valdimarsson, G
影响因子：
5.3
作者：
Agrawal, Smriti A.;Anand, Deepti;Siddam, Archana D.;Kakrana, Atul;Dash, Soma;Scheiblin, David A.;Dang, Christine A.;Terrell, Anne M.;Waters, Stephanie M.;Singh, Abhyudai;Motohashi, Hozumi;Yamamoto, Masayuki;Lachke, Salil A.
通讯作者：
Lachke, Salil A.
DOI：
10.1142/s1793962317500015
发表时间：
2017-03-01
影响因子：
1.2
作者：
Chen, Yong-Chao;Gao, Xin-Bao;Lv, Hui-Miao
通讯作者：
Lv, Hui-Miao