Mangrove sediment, a new source of potential biosurfactant-producing bacteria.
Mangrove sediment, a new source of potential biosurfactant-producing bacteria.
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红树林沉积物，潜在的产生生物表面活性剂的细菌的新来源。
DOI：
10.1007/s13213-012-0424-9
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发表时间：
2012
影响因子：
3
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Saimmai;A.;Tani;A.;Sobhon;V.;& Maneerat;S.
通过以润滑油为唯一碳源的富集培养技术，从泰国南部收集的红树林沉积物样品中分离出产生生物表面活性剂的细菌。总共获得了 1,600 个菌落，通过在含有 1% 不同碳源（商业糖、葡萄糖、糖蜜和废润滑油）的矿物盐培养基中进行定性跌落塌陷试验，对这些菌落进行生物表面活性剂生产的筛选。根据本次测试的结果，95株分离株生物表面活性剂生产呈阳性，其中20株可以降低48小时培养上清液的表面张力。通过16S rRNA基因序列分析评估这20个分离株的系统发育位置。确定了代表八个不同细菌属的菌株的生物表面活性剂的产量。Leucobacter komagatae183 是新分离的显示生物表面活性剂生产的菌株之一，它产生细胞外生物表面活性剂，将培养物上清液的表面张力从 72.0 m/Nm 降低到 32.0 m/Nm。十八个菌株释放细胞外乳化剂，能够稳定形成的乳液。其中，菌株L. komagatae183 和 Ochrobactrum anthropi11/6 表现出与合成表面活性剂相当的乳化活性。总体而言，本研究中分离出的新的生物表面活性剂生产菌株显示出未来在经济有效的工业规模生物技术过程中的开发和使用的有希望的特征。
Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative drop-collapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera.Leucobacter komagatae183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strainsL. komagatae183 andOchrobactrum anthropi11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactant-producing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.