A comparison of bioassay and radioimmunoassay for plasma corticotropin in man.
A comparison of bioassay and radioimmunoassay for plasma corticotropin in man.
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人体血浆促肾上腺皮质激素生物测定法和放射免疫测定法的比较。
DOI：
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发表时间：
1972
影响因子：
5.8
通讯作者：
C. D. West
中科院分区：
文献类型：
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作者：
H. Matsuyama;G. Harada;A. Ruhmann;D. Nelson;C. D. West
以α h_（1 -39）-ACTH或α h-ACTH（Li）为标准品，用生物测定法和放射免疫法测定人血浆ACTH。将每项试验的值转换为另一项试验的值，并计算效价比。双侧肾上腺切除术患者的平均免疫测定与生物测定比值为1.6。接受甲吡酮治疗的受试者的平均比率为2.8，接受心脏直视手术的患者的平均比率为1.8。个体免疫学测定几乎总是高于生物测定，可能是由于存在保留免疫特异性但缺乏生物效力的ACTH片段。
Human plasma ACTH was measured by bioassay and radioimmunoassay, using αh1-39-ACTH or αh-ACTH (Li) as the standard. Values from each assay were converted into the unitage of the other and potency ratios calculated. Patients with bilateral adrenalectomy had a mean immunoassay to bioassay ratio of 1.6. The mean ratio in subjects receiving metyrapone was 2.8, and in patients undergoing open-heart surgery it was 1.8. Individual immunologic determinations were almost always higher than biologic possibly due to the presence of ACTH fragments which retain immunologic specificity but lack biologic potency.