Use of surface-enhanced Raman scattering for detection of cancer-related serum-constituents in gastrointestinal cancer patients
Use of surface-enhanced Raman scattering for detection of cancer-related serum-constituents in gastrointestinal cancer patients
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DOI：
10.1016/j.nano.2013.09.006
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发表时间：
2014-04-01
影响因子：
5.4
通讯作者：
Kudo, Shin-ei
中科院分区：
文献类型：
--
作者：
Ito, Hiroaki;Inoue, Haruhiro;Kudo, Shin-ei
激光介导的表面增强拉曼散射（Sers）具有工业和生物学应用。我们发展了一种快速、简便的方法在磷青铜片表面制备银纳米六方柱（NHC），用于Sers光谱的测量。使用波长为632.8 nm的低强度氦氖红激光束，检测胃癌、结直肠癌或良性疾病患者（各n = 12）的血液样品的Sers光谱;比较患者组之间的Sers光谱强度。良性疾病患者的Sers谱峰高度显著低于胃癌和结直肠癌患者，而胃癌和结直肠癌患者的SERS谱峰高度差异不显著。因此，使用NHC芯片的Sers有望更容易和更快地检测癌症相关血清成分作为生物标志物。在这项研究中，激光介导的表面增强拉曼散射（Sers）被用作胃癌和结直肠癌中癌症相关血清成分的灵敏检测方法，通过在磷青铜芯片表面上使用银纳米级六方柱进行Sers光谱的测量，为开发用于癌症筛查和治疗监测的快速和高通量工具铺平了道路。(C)2014爱思唯尔公司All rights reserved.
Laser-mediated surface-enhanced Raman scattering (SERS) has industrial and biological applications. We have developed a rapid and simple method for generating silver nanoscale hexagonal columns (NHCs) on the surface of a phosphor bronze chip for measurement of SERS spectra. This was used to detect SERS spectra from blood samples obtained from patients with gastric cancer, colorectal cancer, or benign diseases (n = 12 each) using a low intensity helium-neon red laser beam with a 632.8-nm wavelength; the intensity of the SERS spectra was compared among the patient groups. The peak heights of SERS spectra from patients with benign diseases were significantly lower than those from patients with gastric or colorectal cancer, whereas those from patients with gastric cancer and colorectal cancer did not differ significantly. Thus, SERS using NHC chips holds promise for the easier and faster detection of cancer-related serum-constituents as biomarkers.From the Clinical Editor: In this study, laser-mediated surface-enhanced Raman scattering (SERS) was utilized as a sensitive detection method of cancer-related serum-constituents in gastric and colorectal cancer, via the use of silver nanoscale hexagonal columns on the surface of a phosphor bronze chip for measurement of SERS spectra, paving the way to the development of a rapid and high throughput tool for cancer screening and therapy monitoring. (C) 2014 Elsevier Inc. All rights reserved.