THE RNA OF RNASE MRP IS REQUIRED FOR NORMAL PROCESSING OF RIBOSOMAL-RNA
THE RNA OF RNASE MRP IS REQUIRED FOR NORMAL PROCESSING OF RIBOSOMAL-RNA
复制标题
DOI：
10.1073/pnas.91.2.659
复制
发表时间：
1994-01-18
影响因子：
11.1
通讯作者：
LINDAHL, L
中科院分区：
文献类型：
--
作者：
CHU, S;ARCHER, RH;LINDAHL, L
我们已经分离出的克隆补充了我们先前描述的酿酒酵母的rrp 2 -2突变体的温度敏感性和异常rRNA加工模式。DNA测序和限制性酶切分析表明，所有克隆均含有编码核糖核蛋白颗粒RNase MRP的RNA的NME 1基因。缺失分析表明，NME 1基因负责rrp 2 -2表型的互补。在rrp 2突变体中的nme 1基因中鉴定出单个碱基变化，证实RRP 2和NME 1基因是相同的。因此，我们的实验表明，RNase MRP除了先前报道的在线粒体DNA复制的RNA引物形成中的作用外[克莱顿，D. A.（1991）Trends Biochem. Sci. 16，107-111]，参与rRNA加工。
We have isolated clones which complement the temperature sensitivity and abnormal rRNA processing pattern of the rrp2-2 mutant of Saccharomyces cerevisiae we previously described. DNA sequencing and restriction analysis demonstrated that all clones contain the NME1 gene encoding the RNA of the ribonucleoprotein particle RNase MRP. Deletion analysis showed that the NME1 gene is responsible for the complementation of the rrp2-2 phenotype. A single base change was identified in the nme1 gene in the rrp2 mutant, confirming that the RRP2 and NME1 genes are identical. Our experiments therefore indicate that RNase MRP, in addition to its previously reported role in formation of RNA primers for mitochondrial DNA replication [Clayton, D. A. (1991) Trends Biochem. Sci. 16, 107-111], is involved in rRNA processing.